Team:BIT/project hardware

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           <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT/project_hardware&action=edit"></a></div></td>
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      <td class="t2">&nbsp;&nbsp;&nbsp;&nbsp;As we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. <br/>
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   &nbsp;&nbsp;&nbsp;&nbsp;To solve this problem, our sensor has been divided into three, respectively, of <a id="b1" href="https://2013.igem.org/Team:BIT/project_biosensors">hexavalent chromium, tetracycline and β-lactam</a> there is a response, for each sensor, we are all assembled in a downstream of the <a id="b1" href="https://2013.igem.org/Team:BIT/project_AC">amplification block</a> (amplifier) and the <id="b1" href="https://2013.igem.org/Team:BIT/project_AC">control block </a>(controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus <i>lacO</i> and the addition of regular expression <i>lacI</i>, by adjusting the concentration of IPTG, <i>lacO</i> then adjust the boot situation achieved, the final response module for the green fluorescent protein.<br/>
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  &nbsp;&nbsp;&nbsp;&nbsp;In order to achieve real-time detection, we also made a <id="b1" href="https://2013.igem.org/Team:BIT/project_microchip">biochip</a> which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a<a id="b1" href="https://2013.igem.org/Team:BIT/project_hardware"> hand-held electronic equipment</a> made by ourselves.</td>
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          <td><div id="c1"><ul><li><a href="https://2013.igem.org/Team:BIT/project_AC">>>MORE</a></li></ul></div></td>
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          <td><div id="c2"><ul><li><a href="https://2013.igem.org/Team:BIT/project_biosensors">>>MORE</a></li></ul></div></td>
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          <td><div id="c3"><ul><li><a href="https://2013.igem.org/Team:BIT/project_microchip">>>MORE</a></li></ul></div></td>
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          <td><div id="c4"><ul><li><a href="https://2013.igem.org/Team:BIT/project_hardware">>>MORE</a></li></ul></div></td>
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<li><a href="https://2013.igem.org/Team:BIT">HOME</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_biosensors">BIOSENSORS</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_AC">AMPLIFIER</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_microchip">MICRO CHIP</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_hardware">MEASUREMENT</a></li>
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      <td width="814" class="t1"><p class="aaa"><span class="aaa"><span class="aaa">BIT's </span></span><span class="high">measurement</span></p>
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          <p class="high"><span class="aaa"><span class="aaa"><span class="aaa">Based on</span> </span></span><a href="#sbb" class="high">Standard Biomedical Bricks</a></p>
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          <p class="high">&nbsp;</p></td>
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      <td class="t2"><p><span class="high">Measurement</span>:</p>
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        <p>&nbsp; &nbsp; &nbsp;Ok, now we have contributed a new sensor,  put forward a concept of <a href="https://2013.igem.org/Team:BIT/project_AC">controlled amplifier</a> and are working on optimizing it. But we think this is just a star, for we can then use this kind of idea to detect nearly all kinds of antibiotics in the future and we are still trying our best to do so. </p>
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          <p>&nbsp; &nbsp; &nbsp;But a matter is that we cannot see the florescence in normal condition, just like the picture below, we need UV exiting light, narrowband filter and light sensor to tell us the specific intensity of the light, we also need to calculate the material we want to detect using our mathematical model. That’s so complicated.</p></td>
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      <td class="t3"><p><img src="https://static.igem.org/mediawiki/2013/d/d1/BITsam.jpg" alt="" width="350"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Here, a small, cheap and handheld device, together with our <a href="https://2013.igem.org/Team:BIT/project_microchip">micro chip</a> , is produced which together with our biological core, forms a complete detecting system. If you are interested in the detailed structure of our detecting system, please click <a href="http://v.youku.com/v_show/id_XNjI3NDk1ODAw.html">Here</a>.</p>
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            <td width="739"><img src="https://static.igem.org/mediawiki/2013/d/d1/BITsam.jpg" alt="" width="233" height="192"><img src="https://static.igem.org/mediawiki/2013/8/85/BITchi.jpg" alt="" width="233"><img src="https://static.igem.org/mediawiki/2013/c/c7/BITdet.jpg" alt="" width="233" height="195"></td>
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            <td><p class="t2"><img src="https://static.igem.org/mediawiki/2013/c/cd/BITsys.jpg" alt="" width="700"></p></td>
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        <p class="t2"><span class="aaa"><span class="high"><span class="t2">Parameters of the system :</span></span></span></p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Cost: 260RMB/$40 (device &amp; chip). </p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Size: 10cmX6.5cmX5cm</p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Power: 20W Max, 3W Average</p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Functions: User-define mathematical model. Fast detection. Temperature &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;  &nbsp;&nbsp;Control . </p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Resolution: 5 ng/ml Standard fluorescein sodium, 2~5ng/ml Tet.</p>
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<p class="t2">&nbsp; &nbsp; &nbsp;The small device uses normal color sensors and light path similar to UV spectrophotometer, but contains a new, or rather strange mathematical model, a combination of our biological model and relative non-biological equations. </p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/7/77/BITthe.jpg" width="701" height="365"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;For the specific data of the measure system, please click <a href="https://2013.igem.org/Team:BIT/project_data">Here.</a>         </p>
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        <p class="t2">&nbsp;</p>
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        <p class="t2"><span class="high">Standard Biomedical Bricks</span><a name="sbb"></a></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Well, as for biological detection of harmful materials, such as <span class="aaas"><span class="ww">antibiotics, heavy metals, aromatic hydrocarbons, radioactive substances, pesticide residues</span></span> and so on, we need a biological output in <span class="ww">color, fluorescence, conductivity, OD value </span>and many other forms.</p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/4/43/BITaa.gif" width="700" height="500"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;But those outputs may be so weak, just like our fluorescence, without the <a href="https://2013.igem.org/Team:BIT/project_AC" class="ww">controlled amplifier</a>, it can hardly be seen by our eyes, even under UV light.</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Biomedical machines are used in this case, but  those machines can hardly be found out of the lab.</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp; So we put forward <span class="high"><span class="ww">standard biomedical &amp; biological system</span></span>, that is: to design a biological core, finishing the corresponding mathematical model, and a standard biomedical external device. </p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Just like us, our aim is <span class="ww"><span class="aaa"><span class="ww">Practical Application</span></span></span>. We created a biosensor, an amplifier and their mathematical models. </p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;<span class="ww">Standard biomedical bricks</span> means the specific mathematical models which suits for our low cost sensors. iGEM is an application-oriented contest, so detection needs to be economic and convenient. Now we have many standard electronic sensors, BIT iGEM team has also optimized a processor which has an interface suiting for any kinds of sensors.<span class="ww"> What we need to do</span> is to <span class="ww"><span class="t2"><span class="aaa"><span class="ww">build up our engineered bacteria</span></span></span></span>,<span class="ww"> burn the standard mathematical models in the processor</span> and <span class="ww">connect the suitable sensor to the processor</span>. Then a complete and application-oriented system is built. Just like our detecting system, we have made many editions :</p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/f/f6/BITqjf.jpg" width="700" height="470"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Then we arrange user-friendly I/O interface to the standard processor, making a colorful cover to the whole system. That means, you can achieve any kinds of detection using the same device, that sounds cool, isn’t it?</p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/0/06/BITaaa.jpg" width="700" height="442"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;So what we have done at this time is just to simplify the old measurement and provide a new, convenient, economic measurement which detection can be achieved in kitchen. Maybe, some years later, you can see a &quot;bio-antibiotic detector&quot; in supermarket, and let's wait for the coming of that day !</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;We have put forward a concept, and now we have make part of the concept into reality. We will go further on our way to overcome any difficulties, and we eagerly need partners to fight with us. If you have interest in it, please <a href="mailto:hqing@bit.edu.cn;824074989@qq.com">CLICK HERE</a> to contact us.</p>
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