Team:BIT/project hardware

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           <td width="713"><div><a id="a1" href="https://2013.igem.org/wiki/index.php?title=Team:BIT/project_hardware&action=edit"></a></div></td>
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      <td bgcolor="#000000" class="tou">&nbsp;&nbsp;&nbsp;
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        <p>BIT's Measurement </p>
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        <p>&nbsp;  </p>
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          <td><div id="c1"><ul><li><a href="https://2013.igem.org/Team:BIT/project_AC">>>MORE</a></li></ul></div></td>
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        <p>&nbsp;</p>
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          <td><div id="c2"><ul><li><a href="https://2013.igem.org/Team:BIT/project_biosensors">>>MORE</a></li></ul></div></td>
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        <p class="qqq"> <span class="t2"><span class="t1"><span class="nor">Ok , Now we have contributed a new sensor ,  put forward a concept of controlled amplifier and are working on optimizing it .But we think this is just a start ,for we can then use this kind of idea to detect nearly all kinds of antibiotics in the future and we are still trying our best to do so . </span></span></span></p>
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          <td><div id="c3"><ul><li><a href="https://2013.igem.org/Team:BIT/project_microchip">>>MORE</a></li></ul></div></td>
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          <td><div id="c4"><ul><li><a href="https://2013.igem.org/Team:BIT/project_hardware">>>MORE</a></li></ul></div></td>
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<li><a href="https://2013.igem.org/Team:BIT">HOME</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_biosensors">BIOSENSORS</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_AC">AMPLIFIER</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_microchip">MICRO CHIP</a></li>
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<li><a href="https://2013.igem.org/Team:BIT/project_hardware">MEASUREMENT</a></li>
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      <td width="814" class="t1"><p class="aaa"><span class="aaa"><span class="aaa">BIT's </span></span><span class="high">measurement</span></p>
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          <p class="high"><span class="aaa"><span class="aaa"><span class="aaa">Based on</span> </span></span><a href="#sbb" class="high">Standard Biomedical Bricks</a></p>
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          <p class="high">&nbsp;</p></td>
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      <td class="t2"><p><span class="high">Measurement</span>:</p>
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        <p>&nbsp; &nbsp; &nbsp;Ok, now we have contributed a new sensor,  put forward a concept of <a href="https://2013.igem.org/Team:BIT/project_AC">controlled amplifier</a> and are working on optimizing it. But we think this is just a star, for we can then use this kind of idea to detect nearly all kinds of antibiotics in the future and we are still trying our best to do so. </p>
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          <p>&nbsp; &nbsp; &nbsp;But a matter is that we cannot see the florescence in normal condition, just like the picture below, we need UV exiting light, narrowband filter and light sensor to tell us the specific intensity of the light, we also need to calculate the material we want to detect using our mathematical model. That’s so complicated.</p></td>
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      <td class="t3"><p><img src="https://static.igem.org/mediawiki/2013/d/d1/BITsam.jpg" alt="" width="350"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Here, a small, cheap and handheld device, together with our <a href="https://2013.igem.org/Team:BIT/project_microchip">micro chip</a> , is produced which together with our biological core, forms a complete detecting system. If you are interested in the detailed structure of our detecting system, please click <a href="http://v.youku.com/v_show/id_XNjI3NDk1ODAw.html">Here</a>.</p>
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            <td width="636"><img src="https://static.igem.org/mediawiki/2013/5/5e/BITant.jpg" width="600">a</td>
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            <td width="739"><img src="https://static.igem.org/mediawiki/2013/d/d1/BITsam.jpg" alt="" width="233" height="192"><img src="https://static.igem.org/mediawiki/2013/8/85/BITchi.jpg" alt="" width="233"><img src="https://static.igem.org/mediawiki/2013/c/c7/BITdet.jpg" alt="" width="233" height="195"></td>
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            <td width="151" class="nor"><p class="qqq">Antibiotics around</p>
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                <p class="qqq"> the globe</p></td>
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            <td><p class="t2"><img src="https://static.igem.org/mediawiki/2013/c/cd/BITsys.jpg" alt="" width="700"></p></td>
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        <p class="qqq">&nbsp;</p>
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        <p class="t2"><span class="aaa"><span class="high"><span class="t2">Parameters of the system :</span></span></span></p>
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          <p class="qqq">Q:<span class="nor">So ,you have already pur forward a new sensor and a <a href="https://2013.igem.org/Team:BIT/project_AC">controlled amplifier </a>,that's great but I saw a <a href="https://2013.igem.org/Team:BIT/project_microchip">micro chip </a>on your wiki , I wonder what is it used for .</span></p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Cost: 260RMB/$40 (device &amp; chip). </p>
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          <p class="qqq"><span class="ans"><span class="qqq">A:</span></span><span class="nor"> well ,that 's it ! first ,have a look at the picture below :</span></p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Size: 10cmX6.5cmX5cm</p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Power: 20W Max, 3W Average</p>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Functions: User-define mathematical model. Fast detection. Temperature &nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;  &nbsp;&nbsp;Control . </p>
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              <td width="396"><img src="https://static.igem.org/mediawiki/2013/d/d1/BITsam.jpg" width="350"></td>
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            <p class="t2">&nbsp;&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; Resolution: 5 ng/ml Standard fluorescein sodium, 2~5ng/ml Tet.</p>
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              <td width="494" class="nor">This first picture shows that we can hardly see any differences between our samples in normal conditions , the second picture is what we see under UV light , looking through a narrowband filter ,we can get the 3rd picture .</td>
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<p class="t2">&nbsp; &nbsp; &nbsp;The small device uses normal color sensors and light path similar to UV spectrophotometer, but contains a new, or rather strange mathematical model, a combination of our biological model and relative non-biological equations. </p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/7/77/BITthe.jpg" width="701" height="365"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;For the specific data of the measure system, please click <a href="https://2013.igem.org/Team:BIT/project_data">Here.</a>         </p>
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          <p class="nor">&nbsp;&nbsp;&nbsp;&nbsp;but don't you think that 's so hard to detect milk using this kind of method as narrowband filter and UV exiting light are hard to find out of the lab ?</p>
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        <p class="t2">&nbsp;</p>
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          <p class="qqq">&nbsp;&nbsp;&nbsp;&nbsp;<span class="nor">the chip ,together with a electronic device , is a perfect solution to this problem . Just inject the milk and engineering bacteria into the chip </span> <span class="nor">,and put the chip into the electronic device ,wait for some time ,then we can directly read the data on the screen .</span></p>
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        <p class="t2"><span class="high">Standard Biomedical Bricks</span><a name="sbb"></a></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Well, as for biological detection of harmful materials, such as <span class="aaas"><span class="ww">antibiotics, heavy metals, aromatic hydrocarbons, radioactive substances, pesticide residues</span></span> and so on, we need a biological output in <span class="ww">color, fluorescence, conductivity, OD value </span>and many other forms.</p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/4/43/BITaa.gif" width="700" height="500"></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;But those outputs may be so weak, just like our fluorescence, without the <a href="https://2013.igem.org/Team:BIT/project_AC" class="ww">controlled amplifier</a>, it can hardly be seen by our eyes, even under UV light.</p>
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              <td width="344"><img src="https://static.igem.org/mediawiki/2013/9/9a/BITscr.jpg" width="344" height="269"></td>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Biomedical machines are used in this case, but  those machines can hardly be found out of the lab.</p>
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              <td width="46"><img src="https://static.igem.org/mediawiki/2013/2/22/BITrst.jpg" width="368" height="267"></td>
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        <p class="t2">&nbsp; &nbsp; &nbsp; So we put forward <span class="high"><span class="ww">standard biomedical &amp; biological system</span></span>, that is: to design a biological core, finishing the corresponding mathematical model, and a standard biomedical external device. </p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Just like us, our aim is <span class="ww"><span class="aaa"><span class="ww">Practical Application</span></span></span>. We created a biosensor, an amplifier and their mathematical models. </p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;<span class="ww">Standard biomedical bricks</span> means the specific mathematical models which suits for our low cost sensors. iGEM is an application-oriented contest, so detection needs to be economic and convenient. Now we have many standard electronic sensors, BIT iGEM team has also optimized a processor which has an interface suiting for any kinds of sensors.<span class="ww"> What we need to do</span> is to <span class="ww"><span class="t2"><span class="aaa"><span class="ww">build up our engineered bacteria</span></span></span></span>,<span class="ww"> burn the standard mathematical models in the processor</span> and <span class="ww">connect the suitable sensor to the processor</span>. Then a complete and application-oriented system is built. Just like our detecting system, we have made many editions :</p>
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          <p class="qqq">&nbsp;</p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/f/f6/BITqjf.jpg" width="700" height="470"></p>
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           <p class="qqq">Q: <span class="nor">Oh I see , it is just a small Fluorescence microplate reader , right?</span></p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;Then we arrange user-friendly I/O interface to the standard processor, making a colorful cover to the whole system. That means, you can achieve any kinds of detection using the same device, that sounds cool, isn’t it?</p>
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          <p class="qqq">A:<span class="nor">Well ,many people have asked this question . On the one hand , it uses the lightpath similiar to the Fluorescence microplate reader, but on the other hand , it is not just a detector , it is a new concept ,not only for BIT ,but for the whole iGEM.</span></p>
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        <p class="t2"><img src="https://static.igem.org/mediawiki/2013/0/06/BITaaa.jpg" width="700" height="442"></p>
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          <p class="qqq">Q:<span class="nor">That sounds interesting ,so what the concept is ? I wonder how can it help iGEM.</span></p></td>
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        <p class="t2">&nbsp; &nbsp; &nbsp;So what we have done at this time is just to simplify the old measurement and provide a new, convenient, economic measurement which detection can be achieved in kitchen. Maybe, some years later, you can see a &quot;bio-antibiotic detector&quot; in supermarket, and let's wait for the coming of that day !</p>
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        <p class="t2">&nbsp; &nbsp; &nbsp;We have put forward a concept, and now we have make part of the concept into reality. We will go further on our way to overcome any difficulties, and we eagerly need partners to fight with us. If you have interest in it, please <a href="mailto:hqing@bit.edu.cn;824074989@qq.com">CLICK HERE</a> to contact us.</p>
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Latest revision as of 14:14, 28 October 2013

iGEM BIT

     
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