Team:Hong Kong CUHK/futureapp

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   <h2>Human Practice</h2>
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   <h2>Future Application</h2>
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<h3>Future Application</h3>
 
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<p2><p><strong><u>Control enzymatic reactions</strong>
<p2><p><strong><u>Control enzymatic reactions</strong>
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<p><ul>
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   <li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li>
   <li>Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.</li>
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<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/9/96/Sry12.png" alt="1" /></p>
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<p><strong><u>Degradation of cellular proteins</strong>
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  <li>Utilize the Ubiquitin/26S proteasome system</li>
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  <li>Linking the voltage switch with E1/E2/E3 protein</li>
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  <li>Control degradation rate of cellular proteins by controlling ubiquitinylation rate</li>
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<p><strong><u>Study genes using protein complementation</strong>
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  <li>Split genes into two parts, and insert into our system</li>
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  <li>Use voltage switch to control the split and refold of a gene.</li>
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  <li>Possible tool for the screening of new protein complementation compatible proteins.</li>
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<p align="center"><img width="600" height="200" src="https://static.igem.org/mediawiki/2013/f/f3/Sry13.png" alt="2" /></p>
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<p><strong><u>Study Membrane Potential of Organelles</strong>
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<p>Similar to high school talk, majority of high school students do not know what is iGEM or synthetic biology.</p>
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<p>In relation to our project, we review whether they have heard of Polycyclic Aromatic Hydrocarbon or Benzo[a]pyrene.</p>
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<p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/3/3a/Sry2.jpg" alt="2" /></p>
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  <li>Target voltage switch system with BiFC onto the membrane of organelles interested</li>
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<p>Most of the students have not heard of Polycyclic Aromatic Hydrocarbon or benzo alpha pyrene, despite of their hazards.</p>
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  <li>Obtain fluorescent signal</li>
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<p>We investigate their response towards our project ideas.</p>
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  <li>Estimate membrane potential</li>
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<p align="center"><img width="553" height="365" src="https://static.igem.org/mediawiki/2013/a/af/Sry3.jpg" alt="3" /></p>
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<p>Majority of the high school students think that our project idea is interesting, useful, or both.</p>
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<p>Beyond home, religion has been a great issue for synthetic biology. Interestingly,
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50% Hong Kong’ population are irreligious or having other minority beliefs. The correlation between religious beliefs to acceptability of synthetic biology among the high school students is illustrated below. We asked them to rate their degree of acceptability towards synthetic biology within the scale 1 to 5 and we calculate the mean. (1=Not acceptable, 3=Acceptable, 5=Very acceptable)
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<p align="center"><img width="553" height="311" src="http://https://static.igem.org/mediawiki/2013/3/3d/Sry5.jpg" alt="5" /></p>
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<p>The data shows that synthetic biology is slightly more acceptable among non-believers. But the mean values lie above 3 for both views based on religious beliefs and personal opinion, thus synthetic biology is well accepted by our respondents.
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Lastly, we assess their response on the comic.
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<p align="center"><img width="553" height="285" src="https://static.igem.org/mediawiki/2013/1/1d/Sry6.jpg" alt="6" /></p>
 
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<p>The comic is interesting for high school students as most of them rate it as 3 or above.</p>
 
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<p>&nbsp;</p>
 
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<p align="center"><img width="553" height="407" src="https://static.igem.org/mediawiki/2013/d/de/Sry7.jpg" alt="7" /></p>
 
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<p>Our comic delivers the content well, 97% of our respondents rate it as 3 or above.</p>
 
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<p align="center"><img width="552" height="351" src="https://static.igem.org/mediawiki/2013/0/04/Sry8.jpg" alt="8" /></p>
 
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<p>After reading the comic, most of the high school students have a better understanding on iGEM, synthetic biology or both.</p>
 
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<p>Parents (24 parents)</p>
 
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<p>During this open day event, we got the chance to meet some parents and conduct a survey. As most of them actually came to check on their children potential field of study in CUHK, we are so happy to learn that they are also enthusiastic about iGEM and synthetic biology.</p>
 
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<p>We asked them on their familiarity to synthetic biology.</p>
 
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<p align="center"><img src="https://static.igem.org/mediawiki/2013/6/6e/Sry9.jpg" alt="9" width="555" height="320" /> </p>
 
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<p>This result is consistent with response of high school students that came to our talk. (for question: do your parents understand what synthetic biology is?)</p>
 
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<p>Parents are the gatekeepers of purchase, thus we investigate on their willing ness to buy and use products that is produced from synthetic biology.</p>
 
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  <p align="center"><img width="553" height="320" src="https://static.igem.org/mediawiki/2013/9/93/Sry10.jpg" alt="10" /></p>
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<p>It is quite surprising that parents are willing to buy or use products from synthetic biology. They probably think that what has been in the market is safe to use.</p>
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<p>We also explore their acceptability on using bacteria in daily lives. For example, using microorganism to remove cancer-causing substances from environment. This is to know whether they will welcome our project or not in the future.</p>
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<p align="center"><img width="554" height="413" src="https://static.igem.org/mediawiki/2013/6/60/Sry11.jpg" alt="11" /></p>
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<p>Most of the parents rated 3 for their acceptability, which means they accept synthetic biology but neither very supportive nor against it.</p>
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<strong><p>Conclusion</p></strong>
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<p>From the data that we got, many people still left uninformed about what is iGEM and synthetic biology. This is the same case for  hazards of Polycyclic Aromatic Hydrocarbon and Benzo[a]pyrene. Our project idea is well received by our audiences.
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<p>One’s religious belief affects the way a person responds to the idea of synthetic biology. Non-believers most welcome synthetic biology; there is no need to hesitate to make iGEM and synthetic biology widespread in Hong Kong and Macau as most of them are non-believers.</p><p>Parents’ of high school students mostly do not know what synthetic biology is. Most of the parents are welcome to purchase and use products from synthetic biology. We have to start approaching these adults with the idea of synthetic biology, then they can educate their youngsters about it. Lastly, many agree that synthetic biology will be an integral part of a knowledge-based economy in the future, for Hong Kong and Macau.</p>
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<p>Information two-way flow from surrounding to individual can be mediated through our comic. As our data shown that surrounding affects an individual (ex. Religion) and an individual understanding about an important topic like synthetic biology may contribute to the way he/she sees the surrounding situation, like the economy structure preferred.</p>
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Latest revision as of 18:09, 28 October 2013

iGEM CUHK

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