Team:UCSF/Project/Conjugation/Promoter

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<font face="calibri" size = "2"> Figure 3. (A) Sequence of different lactose inducible promoters. Yellow rectangle indicates -35 and -10 core elements of the promoter, red character indicates transcription start site, and lac operators are shown in blue boxes. (B) Sequences of three lac operators found in E. coli genome. Bases listed with capital letters are identical to bases in the primary LacO1.  
<font face="calibri" size = "2"> Figure 3. (A) Sequence of different lactose inducible promoters. Yellow rectangle indicates -35 and -10 core elements of the promoter, red character indicates transcription start site, and lac operators are shown in blue boxes. (B) Sequences of three lac operators found in E. coli genome. Bases listed with capital letters are identical to bases in the primary LacO1.  
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<font face="calibri" size = "2"> Figure 4. Impact of DNA looping on lac repressor binding to DNA. (A) Minimum energy configurations of DNA fragments complexed with the LacR tetramer (From Swigon et al., 2006). (B) DNA looping would change the kinetic behavior of pLAC promoter. Scheme of promoters (up) and induction results (down) of pLAC with (right) and without (left) DNA looping (From Oehler et al., 2006).
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Revision as of 18:59, 28 October 2013


CRISPRi Circuit: Promoter Design and Engineering

After our initial promoter assays our goal was to create engineered versions of our promoters responsive to high and low levels of inducer, and here we’ve chosen pLAC promoter as proof of concept.

The lactose inducible promoter pLAC, first discovered in Escherichia coli and serving as a core component of lac operon responsible for lactose metabolism, is one of the best-studied and engineered prokaryotic transcriptional regulatory system. The switch like behavior of this promoter in response to lactose level is achieved through interaction of a regulatory protein LacI and small fragments of DNA sequence, named lac operators, within the promoter region.

The LacI protein monomers self-associate into an unusual tetramer that appears roughly as a V-shaped dimer of dimers (See Figure 1A for detailed information), and could be further divided into four discrete functional units: a N-terminal headpiece with a helix-turn-helix motif capable of binding to the DNA, a hinge region connecting headpiece with core, and the protein core with a N-terminal lactose binding domain and a C-terminal helix responsible for dimerization. Each dimeric repressor is capable of binding to a 21-base-pair duplex deoxyoligonucleotides, the lac operator site. (Wilson et al., 2007; Lewis, 2011) Meanwhile, the lac operator sequence is a pseudo-symmetric DNA sequence that was first identified to be a 27-base-pair section (lacO1) (Gilbert et al., 1973) and further narrowed down to around 17 base pairs for minimal specific binding requirement (Bahl et al., 1977) (Figure 1B).

Figure 1. Scheme of lac repressor and operator site. (A) A ribbon diagram of the quaternary structure of the lac repressor complexed to DNA. Lac repressor is a tetrameric structure where each monomer is drawn in color (blue/grey for left dimer and green/pink for right dimer). (From Wilson et al., 2007) (B) The 27-base-pair deoxyoligonucleotide first identified as the operator region of the lactose operon (LacO1). Bold characters showed the 17 base pairs required for specific binding.
In the absence of lactose, the repressor protein binds to lac operator with a high affinity, and therefore compromises RNA polymerase binding, elongation and/or initiation. While in the presence of lactose and a variety of other gratuitous inducers (e.g. isopropyl-β,D-thiogalactoside, IPTG), theses “inducers” binds with high affinity to lac repressors and lower its affinity for the operator sequence, thereby allows the transcription of downstream mRNA (Figure 2).
Figure 2. Scheme of the lactose operon and its switch-like behavior. The lacZ, lacY, lacA genes are lactose metabolic genes that located downstream of the pLAC promoter.
Apart from the original pLAC promoter derived from nature, various engineering effort have been made for the optimization of its expression and induction properties and therefore facilitates its usage in synthetic biological systems (i.e. pTAC (de Boer et al., 1983), pLlacO_1 (Lutz and Bujard, 1997), etc., See Figure 3A for detailed information). Meanwhile, in addition to the first identified lacO1 operator section, two auxiliary pseudo-operators, lacO2 (Reznicoff et al., 1974) and lacO3 (Pfahl et al., 1979) sites, were identified downstream (lacO2) or upstream (lacO3) of the promoter region with similar sequences (Figure 3B). These operators were later shown to be required for maximal repression, for the fact that tetrameric lac repressor was ideally suited to bind two operators simultaneously, and therefore caused so-called “repression loops” (Figure 4A) (Mossing et al., 1986; Oehler et al., 1990). Studies have also shown that this DNA looping would also change the kinetic behavior (i.e. activation coefficient) of the promoter (Figure 4B) (Oehler et al., 2006).
Figure 3. (A) Sequence of different lactose inducible promoters. Yellow rectangle indicates -35 and -10 core elements of the promoter, red character indicates transcription start site, and lac operators are shown in blue boxes. (B) Sequences of three lac operators found in E. coli genome. Bases listed with capital letters are identical to bases in the primary LacO1.
Figure 4. Impact of DNA looping on lac repressor binding to DNA. (A) Minimum energy configurations of DNA fragments complexed with the LacR tetramer (From Swigon et al., 2006). (B) DNA looping would change the kinetic behavior of pLAC promoter. Scheme of promoters (up) and induction results (down) of pLAC with (right) and without (left) DNA looping (From Oehler et al., 2006).

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