"
Team:Penn/MethylaseOverview
From 2013.igem.org
(Difference between revisions)
| Line 34: | Line 34: | ||
Now that the MaGellin assay was validated for non-specific methylases, we were ready to test site-specific methylases. Our MaGellin assay is ideal for high-throughput construction and testing of these enzymes. Site-specific methylases are fusion proteins, a DNA binding domain linked to a methylase by a serine glycine chain. They can direct DNA methylation to specific sequences, likely promoter regions for use as a transcriptional silencer. We used the prokaryotic methylase M.SssI for all of our studies (BBa_K1128000). | Now that the MaGellin assay was validated for non-specific methylases, we were ready to test site-specific methylases. Our MaGellin assay is ideal for high-throughput construction and testing of these enzymes. Site-specific methylases are fusion proteins, a DNA binding domain linked to a methylase by a serine glycine chain. They can direct DNA methylation to specific sequences, likely promoter regions for use as a transcriptional silencer. We used the prokaryotic methylase M.SssI for all of our studies (BBa_K1128000). | ||
<p>We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize our two novel site-specific methylases: using the TALE and CRISPR/Cas binding domains. | <p>We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize our two novel site-specific methylases: using the TALE and CRISPR/Cas binding domains. | ||
| - | </br></br> | + | </br></br> |
| - | + | ||
| + | <center><a href="https://2013.igem.org/Team:Penn/Software">←Previous</a> <a href="https://2013.igem.org/Team:Penn/MethylaseCharacterization">Next→</a></center> | ||
Revision as of 20:14, 28 October 2013
Site-Specific Methylases
We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize our two novel site-specific methylases: using the TALE and CRISPR/Cas binding domains.
