Team:DTU-Denmark/Notebook/18 July 2013

From 2013.igem.org

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(Procedure)
(Procedure)
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- made gel for yesterday's PCR
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- made gel for yesterday's PCR of Nir operon -> empty
- USER reaction with HAO + pZA21 and AMO + pZA21
- USER reaction with HAO + pZA21 and AMO + pZA21

Revision as of 14:48, 18 July 2013


Contents

208


Main purpose


  • USER reaction

Who was in the lab


Gosia, Henrike, Julia

Procedure


- made gel for yesterday's PCR of Nir operon -> empty

- USER reaction with HAO + pZA21 and AMO + pZA21

- nandrop measurement of yesterday's miniprep showed it had very small yield, so we will redo it

- inocculated cultures for miniprep of RFP in pZA21 and cycAX in pZA21