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| - | <a href="https://2013.igem.org/Team:Tianjin/Project">Background</a>
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| - | </li>
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Design">Design</a>
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project">Experiment</a>
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment">System construction and optimization</a>
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Endo-Induce">Endogenous Alkanes Induce</a>
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/Exo-Induce">Exogenous Alkanes Induce</a>
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| - | <a href="https://2013.igem.org/Team:Tianjin/Project/Experiment/TetA">TetA</a>
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| - | <center><span style="font-family:Arial;font-size:46px;color:#000;"> Experiment</span></center>
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| - | =Endogenous Induce&Resistance Selection=
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| - | <p>Here, we want to test the resistance against tetracycline of Alk-Sensor induced by endogenous alkanes.</p>
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| - | <p>We put the alkane producing module into cells to test the function of TetA:</p>
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| - | <div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/d/db/Tju-p-1.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/d/db/Tju-p-1.jpg" width="500px" /></a></div>
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| - | <p><b>Construct:</b></p>
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| - | <p>Left: No.60: TetA downstream of Palkm and AlkR downstream of J23103, are cloned into vector PSB1C3 and there’s a alkane producing vector PSB3K3.</p>
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| - | <p>Right: No.61: We construct a blank vector PSB3K3 without NPDC and AAR as blank control, and the other vector PSB1C3 is the same as No.60.</p>
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| - | <p><b>Characterization: </b></p>
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| - | <p>Strains of No.60 are cultured in LB medium for about 12 hours. Add 17μg/mL Kan, 12μg/mL Cm and 12μg/mL Tc respectively. After that, inoculate into 3 ml LB medium for an overnight cultures at 37 ℃ with 220rpm shaking.</p>
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| - | <div style="text-align:center;vertical-align:middle;"><a href="#" target="_blank" ><img src="#" width="500px" /></a></div>
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| - | <p><b>Result:</b> </p>
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| - | <p>Alkane producing E.coli can grow in tube but blank vector E.coli cannot grow .</p>
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| - | <p><b>Conclusion:</b></p>
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| - | <p>Synthesized alkanes induce the expression of alkR, which enables E.coli to survive under the pressure of Tetracycline.</p>
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| - | <p>Thus, we can get a 3×3 matrix, with promoter strength from weak, medium to strong, and plasmid copy number from small, medium to large.</p>
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| - | <div style="text-align:center;vertical-align:middle;"><a href="https://static.igem.org/mediawiki/2013/5/57/Tju-p-2.jpg" target="_blank" ><img src="https://static.igem.org/mediawiki/2013/5/57/Tju-p-2.jpg" width="720px" /></a></div>
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| - | E-mail：austinamens@gmail.com | Address:Building No.20, No.92 Weijin Road, Tianjin, China | Zip-code：300072
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| - | <br />Copyright 2013 © Tianjin University iGEM Team
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