Team:Cornell/notebook/plasmids
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+ | <div class="row team-bios-container"> | ||
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+ | <h2 class="centered">Plasmids</h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | <table class="tableizer-table" rules="all"> | ||
<tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th><th>Antifungal Resistance</th></tr> | <tr class="tableizer-firstrow"><th>Plasmid Name</th><th>Part</th><th>Contents/Description</th><th>Origin</th><th>Backbone</th><th>Antibiotic Resistance</th><th>Antifungal Resistance</th></tr> | ||
- | <tr><td>pC13A</td><td>BBa_K523022</td><td> | + | <tr><td>pC13A</td><td>BBa_K523022</td><td>crtEBI</td><td>Kit Plate 1 8P</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr> |
<tr><td>pC13B</td><td>BBa_K539119</td><td>crtY</td><td>Kit Plate 1 12K</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr> | <tr><td>pC13B</td><td>BBa_K539119</td><td>crtY</td><td>Kit Plate 1 12K</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr> | ||
<tr><td>pA13C</td><td>pIVEX2.3d</td><td>T7 promoter/terminator</td><td>Luo Lab</td><td>pIVEX2.3d</td><td>Ampicillin</td><td>N/A</td></tr> | <tr><td>pA13C</td><td>pIVEX2.3d</td><td>T7 promoter/terminator</td><td>Luo Lab</td><td>pIVEX2.3d</td><td>Ampicillin</td><td>N/A</td></tr> | ||
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<tr><td>pC13DR</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI</td><td>DQ, DO</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr> | <tr><td>pC13DR</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI</td><td>DQ, DO</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr> | ||
<tr><td>pC13DS</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII</td><td>DS, DR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr> | <tr><td>pC13DS</td><td>unsubmitted</td><td>Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII</td><td>DS, DR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr> | ||
+ | `<tr><td>pC13DT</td><td>unsubmitted</td><td>T7 + crtEBI</td><td>A, D</td><td>pSB1C3</td><td>Chloramphenicol</td><td>N/A</td></tr> | ||
+ | <tr><td>pC13DU</td><td>unsubmitted</td><td>Lhom + PtrpC + nptIIBB + Rhom</td><td>BK, BM, Y</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr> | ||
+ | <tr><td>pC13DV</td><td>unsubmitted</td><td>Lhom + PgpdA + nptIIBB + Rhom</td><td>BK, BM ,BQ</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Geneticin</td></tr> | ||
+ | <tr><td>pC13DW</td><td>unsubmitted</td><td>Lhom + PgpdA + hphBB + Rhom</td><td>BK, BM, BR</td><td>pSB1C3</td><td>Chloramphenicol</td><td>Hygromycin</td></tr> | ||
+ | <tr><td>pS13DX</td><td>invalid</td><td>Lhom + PtrpC + nptIIBB + Rhom</td><td>BK, BM, Y</td><td>pOSCAR</td><td>Spectinomycin</td><td>Geneticin</td></tr> | ||
+ | <tr><td>pS13DY</td><td>invalid</td><td>Lhom + PgpdA + nptIIBB + Rhom</td><td>BK, BM ,BQ</td><td>pOSCAR</td><td>Spectinomycin</td><td>Geneticin</td></tr> | ||
+ | <tr><td>pS13DZ</td><td>invalid</td><td>Lhom + PgpdA + hphBB + Rhom</td><td>BK, BM, BR</td><td>pOSCAR</td><td>Spectinomycin</td><td>Hygromycin</td> | ||
+ | <tr><td>pAh13EA</td><td>invalid</td><td>hph + C. heterostrophus Pgpd</td><td>BK, BM, BR</td><td>pHPVEL1</td><td>Spectinomycin</td><td>Hygromycin</td> | ||
+ | <tr><td>pS13EB</td><td>invalid</td><td>pOSCAR backbone for transformation into agrobacterium</td><td>BK, BM, BR</td><td>pOSCAR</td><td>Spectinomycin</td><td>Hygromycin</td> | ||
</table> | </table> | ||
+ | </div> | ||
+ | </html> |
Latest revision as of 03:17, 29 October 2013
Plasmids
Plasmid Name | Part | Contents/Description | Origin | Backbone | Antibiotic Resistance | Antifungal Resistance |
---|---|---|---|---|---|---|
pC13A | BBa_K523022 | crtEBI | Kit Plate 1 8P | pSB1C3 | Chloramphenicol | N/A |
pC13B | BBa_K539119 | crtY | Kit Plate 1 12K | pSB1C3 | Chloramphenicol | N/A |
pA13C | pIVEX2.3d | T7 promoter/terminator | Luo Lab | pIVEX2.3d | Ampicillin | N/A |
pAK13D | BBa_I712074 | T7 promoter | Kit Plate 5 6N | pSB1AK8 | Ampicillin | N/A |
pC13E | BBa_K145001 | T7 polymerase | Kit Plate 4 2C | pSB1C3 | Chloramphenicol | N/A |
pC13F | BBa_J04450 | mRFP in pSB1C3 | T.E. Kit | pSB1C3 | Chloramphenicol | N/A |
pAh13G | pUCATPH | PtrpC, hph, TtrpC | Turgeon lab | pUCATPH | Ampicillin | Hygromycin |
pAg13H | pNG | Pgpd (C. h.), nptII | Turgeon lab | pNG | Ampicillin | Geneticin |
pC13I | BBa_K1021000 | crtE | A | pSB1C3 | Chloramphenicol | N/A |
pC13J | invalid | rbs + crtE | A | pSB1C3 | Chloramphenicol | N/A |
pC13K | BBa_K118003 | crtI | Kit Plate 3 2B | pSB1C3 | Chloramphenicol | N/A |
pC13L | BBa_K118005 | rbs + crtI | Kit Plate 4 4A | pSB1C3 | Chloramphenicol | N/A |
pC13M | BBa_K118002 | crtB | Kit Plate 3 17N | pSB1C3 | Chloramphenicol | N/A |
pC13N | BBa_K118006 | rbs + crtB | Kit Plate 3 17P | pSB1C3 | Chloramphenicol | N/A |
pC13O | BBa_I742155 | rbs + crtY | Kit Plate 5 17P | pSB1C3 | Chloramphenicol | N/A |
pC13P | BBa_K1021007 | PtrpC | G | pSB1C3 | Chloramphenicol | N/A |
pC13Q | BBa_K1021008 | TtrpC | G | pSB1C3 | Chloramphenicol | N/A |
pCg13R | invalid | nptII | H | pSB1C3 | Chloramphenicol | Geneticin |
pCg13S | BBa_K1021001 | nptIIBB | R | pSB1C3 | Chloramphenicol | Geneticin |
pAb13T | pBARGPE1 | PtrpC, bar, TtrpC, PgpdA, lox | Fungal Genetics Stock Center | pBARGPE1 | Ampicillin | Phosphonothricin |
pCg13U | invalid | nptIIBB + TtrpC | S, Q | pSB1C3 | Chloramphenicol | Geneticin |
pCh13V | invalid | hph | G, F | pSB1C3 | Chloramphenicol | Hygromycin |
pCh13W | BBa_K1021002 | hphBB | V | pSB1C3 | Chloramphenicol | Hygromycin |
pCp13X | BBa_K1021003 | bar | T, F | pSB1C3 | Chloramphenicol | Phosphonothricin |
pCg13Y | BBa_K1021015 | PtrpC + nptIIBB | P, S | pSB1C3 | Chloramphenicol | Geneticin |
pC13Z | BBa_K082003 | GFP(+LVA) | Kit Plate 3 20N | pSB1C3 | Chloramphenicol | N/A |
pAK13AA | invalid | PT7 + crtE | D, A | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13AB | invalid | PT7 + crtB | D, M | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13AC | invalid | PT7 + crtI | D, K | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13AD | invalid | PT7 + crtY | D, B | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pC13AE | BBa_K1021016 | PT7 + crtE | F, AA | pSB1C3 | Chloramphenicol | N/A |
pC13AF | BBa_K1021017 | PT7 + crtB | F, AB | pSB1C3 | Chloramphenicol | N/A |
pC13AG | BBa_K1021018 | PT7 + crtI | F, AC | pSB1C3 | Chloramphenicol | N/A |
pC13AH | BBa_K1021019 | PT7 + crtY | F, AD | pSB1C3 | Chloramphenicol | N/A |
pC13AI | BBa_J61046 | lox site | Kit Plate 3 3L | pSB1C3 | Chloramphenicol | N/A |
pA13AJ | BBa_J61046 | lox site | Kit Plate 5 24B | pSB1A2 | Ampicillin | N/A |
pC13AK | BBa_E1010 | mRFP cds only | Kit Plate 3 12N | pSB1C3 | Chloramphenicol | N/A |
pK13AL | BBa_E1010 | mRFP cds only | Kit Plate 5 18F | pSB2K3 | Kanamycin | N/A |
pA13AM | BBa_J23100 | strongest Anderson constitutive promoter with mRFP downstream | Kit Plate 5 18C | BBa_J61002 | Ampicillin & Kanamycin | N/A |
pAK13AN | invalid | PT7+GFP | D, Z | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13AO | invalid | PT7+mRFP | D, AK/AL | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13AP | invalid | PT7+bar | D, X | pSB1AK8 | Ampicillin & Kanamycin | Phosphonothricin |
pC13AQ | BBa_K1021020 | PT7+GFP | AN, F | pSB1C3 | Chloramphenicol | N/A |
pC13AR | BBa_K1021021 | PT7+mRFP | AO, F | pSB1C3 | Chloramphenicol | N/A |
pC13AS | BBa_K1021022 | PT7+bar | AP, F | pSB1C3 | Chloramphenicol | Phosphonothricin |
pC13AT | BBa_K1021023 | PtrpC+GFP | P, Z | pSB1C3 | Chloramphenicol | N/A |
pC13AU | BBa_K1021024 | PtrpC+mRFP | P, AK/AL | pSB1C3 | Chloramphenicol | N/A |
pC13AV | BBa_K1021025 | PtrpC+bar | P, X | pSB1C3 | Chloramphenicol | Phosphonothricin |
pA13AW | invalid | Anderson promoter + T7Pol | AM, E | BBa_J61002 | Ampicillin | N/A |
pK13AX | invalid | Anderson promoter + T7Pol | AW, AL (backbone) | pSB2K3 | Kanamycin | N/A |
pC13AY | unsubmitted | PtrpC+T7Pol | P, E | pSB1C3 | Chloramphenicol | N/A |
pC13AZ | BBa_K1021026 | Anderson promoter + T7Pol | AW, F | pSB1C3 | Chloramphenicol | N/A |
pC13BA | BBa_K1021004 | afp1 | G-block | pSB1C3 | Chloramphenicol | N/A |
pAK13BB | invalid | PT7+nptIIBB | D, S | pSB1AK8 | Ampicillin & Kanamycin | Geneticin |
pC13BC | BBa_K1021032 | PT7+nptIIBB | BB, F | pSB1C3 | Chloramphenicol | Geneticin |
pC13BD | unsubmitted | PtrpC+crtI | P, K | pSB1C3 | Chloramphenicol | N/A |
pA13BE | pHNU3pelA | PpelA, flanked by KpnI/BamHI | Turgeon lab | pHPG | Ampicillin | N/A |
pC13BF | BBa_K1021009 | PpelA | BE, F | pSB1C3 | Chloramphenicol | N/A |
pC13BG | BBa_K1021010 | A. nidulans PgpdA | T, F | pSB1C3 | Chloramphenicol | N/A |
pC13BH | invalid | G. lucidum PgpdA | Ganoderma genome, F | pSB1C3 | Chloramphenicol | N/A |
pC13BI | unsubmitted | G. lucidum PgpdA (BB) | BH | pSB1C3 | Chloramphenicol | N/A |
pC13BJ | BBa_K1021011 | 100bp left region of homology (Ganoderma gpd gene) | Ganoderma genome, F | pSB1C3 | Chloramphenicol | N/A |
pC13BK | BBa_K1021012 | 200bp left region of homology (Ganoderma gpd gene) | Ganoderma genome, F | pSB1C3 | Chloramphenicol | N/A |
pC13BL | BBa_K1021013 | 100bp right region of homology (Ganoderma gpd gene) | Ganoderma genome, F | pSB1C3 | Chloramphenicol | N/A |
pC13BM | BBa_K1021014 | 200bp right region of homology (Ganoderma gpd gene) | Ganoderma genome, F | pSB1C3 | Chloramphenicol | N/A |
pC13BN | unsubmitted | lox [cutsite 1] [cutsite 2] lox | AI | pSB1C3 | Chloramphenicol | N/A |
pC13BO | abandoned | short homology regions flanking two unique cutsites | BJ, BL | pSB1C3 | Chloramphenicol | N/A |
pC13BP | abandoned | long homology regions flanking two unique cutsites | BK, BM | pSB1C3 | Chloramphenicol | N/A |
pC13BQ | BBa_K1021027 | A. nidulans PgpdA + nptIIBB | BG, S | pSB1C3 | Chloramphenicol | Geneticin |
pC13BR | BBa_K1021028 | A. nidulans PgpdA + hphBB | BG, W | pSB1C3 | Chloramphenicol | Hygromycin |
pC13BS | unsubmitted | G. lucidum PgpdABB + nptIIBB | BI, S | pSB1C3 | Chloramphenicol | Geneticin |
pC13BT | unsubmitted | G. lucidum PgpdABB + hphBB | BI, W | pSB1C3 | Chloramphenicol | Hygromycin |
pAK13BU | invalid | PT7 + hphBB | D, W | pSB1AK8 | Ampicillin & Kanamycin | Hygromycin |
pC13BV | BBa_K1021033 | PT7 + hphBB | BU, F | pSB1C3 | Chloramphenicol | Hygromycin |
pA13BW | pBS185CMV-Cre | pBS185 CMV Cre | Addgene | pBS185 | Ampicillin | N/A |
pC13BX | BBa_K1021005 | Cre recombinase | BW | pSB1C3 | Chloramphenicol | N/A |
pC13BY | unsubmitted | PpelA + Cre | BF, BX | pSB1C3 | Chloramphenicol | N/A |
pC13BZ | unsubmitted | PpelA + GFP | BF, Z | pSB1C3 | Chloramphenicol | N/A |
pC13CA | unsubmitted | PpelA + mRFP | BF, AL | pSB1C3 | Chloramphenicol | N/A |
pC13CB | unsubmitted | PT7 + GFP + PtrpC + nptIIBB | Y, AQ | pSB1C3 | Chloramphenicol | Geneticin |
pC13CC | BBa_K1021029 | PtrpC + nptIIBB + TtrpC | Y, Q | pSB1C3 | Chloramphenicol | Geneticin |
pC13CD | BBa_K1021030 | PtrpC + hphBB | P, W | pSB1C3 | Chloramphenicol | Hygromycin |
pC13CE | BBa_K1021034 | PtrpC + hphBB +TtrpC | CD, Q | pSB1C3 | Chloramphenicol | Hygromycin |
pC13CF | unsubmitted | A. n. PgpdA + GFP | BG, Z | pSB1C3 | Chloramphenicol | N/A |
pC13CG | BBa_K1021031 | A. n. PgpdA + mRFP | BG, AK/AL | pSB1C3 | Chloramphenicol | N/A |
pC13CH | unsubmitted | G. l. PgpdA + GFP | BH, Z | pSB1C3 | Chloramphenicol | N/A |
pC13CI | unsubmitted | G. I. PgpdA + mRFP | BH, AK/AL | pSB1C3 | Chloramphenicol | N/A |
pC13CJ | BBa_I742111 | Limonene synthase | Kit plate 4 | pSB1C3 | Chloramphenicol | N/A |
pAK13CK | unsubmitted | PT7+Limonene Synthase | CJ, D | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pC13CL | BBa_K1021006 | Cht1_2 in pSB1C3 | IDT Geneblock | pSB1C3 | Chloramphenicol | N/A |
pC13CM | unsubmitted | PpelA + Cht1_2 | CL, BF | pSB1C3 | Chloramphenicol | N/A |
pS13CN | BBa_K112306 | Lambda Holin | Kit plate 3 2012 BBa_K112306 | BBa_K112950 | Spectinomycin | N/A |
pC13CO | unsubmitted | Lambda Holin in pSB1C3 | CN | pSB1C3 | Chloramphenicol | N/A |
pC13CP | unsubmitted | PpelA+Lambda Holin | CO | pSB1C3 | Chloramphenicol | N/A |
pAK13CQ | invalid | PT7 + rbs + crtE | D, J | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13CR | invalid | PT7 + rbs + crtB | D, N | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13CS | invalid | PT7 + rbs + crtI | D, L | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pAK13CT | invalid | PT7 + rbs + crtY | D, O | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pC13CU | unsubmitted | PT7 + rbs + crtE | F, CQ | pSB1C3 | Chloramphenicol | N/A |
pC13CV | unsubmitted | PT7 + rbs + crtB | F, CR | pSB1C3 | Chloramphenicol | N/A |
pC13CW | unsubmitted | PT7 + rbs + crtI | F, CS | pSB1C3 | Chloramphenicol | N/A |
pC13CX | unsubmitted | PT7 + rbs + crtY | F, CT | pSB1C3 | Chloramphenicol | N/A |
pAK13CY | invalid | PT7 + rbs + GFP | D, DB | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pA13CZ | BBa_B0034 | RBS (strong) | Kit Plate 5, Well 2M | pSB1A2 | Ampicillin | N/A |
pAK13DA | invalid | PT7 + rbs + mRFP | D, DC | pSB1AK8 | Ampicillin & Kanamycin | N/A |
pA13DB | invalid | RBS+GFP | CZ, Z | pSB1A2 | Ampicillin | N/A |
pA13DC | invalid | RBS+mRFP | CZ, AK | pSB1A2 | Ampicillin | N/A |
pC13DD | unsubmitted | PT7+Limonene Synthase | F, CK | pSB1C3 | Chloramphenicol | N/A |
pC13DE | unsubmitted | lox-PtrpC-nptIIBB-lox | Y, BN | pSB1C3 | Chloramphenicol | Geneticin |
pC13DF | unsubmitted | lox-A.PgpdA-nptIIBB-lox | BN, BQ | pSB1C3 | Chloramphenicol | Geneticin |
pC13DG | unsubmitted | lox-A.PgpdA-hphBB-lox | BN, BR | pSB1C3 | Chloramphenicol | Hygromycin |
pC13DH | unsubmitted | lox-PtrpC-hphBB-lox | BN, CD | pSB1C3 | Chloramphenicol | Hygromycin |
pC13DI | unsubmitted | PtrpC + hphBB + GFP | CD, Z | pSB1C3 | Chloramphenicol | Hygromycin |
pC13DJ | unsubmitted | PT7 + crtB + PT7 + crtI | AF, AG | pSB1C3 | Chloramphenicol | N/A |
pC13DK | unsubmitted | PT7 + crtE + PT7 + crtB + PT7 + crtI | DJ, AE | pSB1C3 | Chloramphenicol | N/A |
pC13DL | unsubmitted | PT7 + crtE + PT7 + crtB + PT7 + crtI + PT7 + nptIIBB | DK, BC | pSB1C3 | Chloramphenicol | Geneticin |
pC13DM | unsubmitted | PT7 + rbs + crtB + PT7 + rbs + crtI | CV, CW | pSB1C3 | Chloramphenicol | N/A |
pC13DN | unsubmitted | PT7 + rbs + crtE + PT7 + rbs + crtB + PT7 + rbs + crtI | DM, CU | pSB1C3 | Chloramphenicol | N/A |
pC13DO | unsubmitted | Gan. PgpdA + crtE | BI, I | pSB1C3 | Chloramphenicol | N/A |
pC13DP | unsubmitted | Asp. PgpdA + crtB | BG, M | pSB1C3 | Chloramphenicol | N/A |
pC13DQ | unsubmitted | Asp. PgpdA + crtB + PtrpC + crtI | DP, BD | pSB1C3 | Chloramphenicol | N/A |
pC13DR | unsubmitted | Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI | DQ, DO | pSB1C3 | Chloramphenicol | N/A |
pC13DS | unsubmitted | Gan. PgpdA + crtE + Asp. PgpdA + crtB + PtrpC + crtI + PtrpC + nptII | DS, DR | pSB1C3 | Chloramphenicol | Geneticin |
pC13DT | unsubmitted | T7 + crtEBI | A, D | pSB1C3 | Chloramphenicol | N/A |
pC13DU | unsubmitted | Lhom + PtrpC + nptIIBB + Rhom | BK, BM, Y | pSB1C3 | Chloramphenicol | Geneticin |
pC13DV | unsubmitted | Lhom + PgpdA + nptIIBB + Rhom | BK, BM ,BQ | pSB1C3 | Chloramphenicol | Geneticin |
pC13DW | unsubmitted | Lhom + PgpdA + hphBB + Rhom | BK, BM, BR | pSB1C3 | Chloramphenicol | Hygromycin |
pS13DX | invalid | Lhom + PtrpC + nptIIBB + Rhom | BK, BM, Y | pOSCAR | Spectinomycin | Geneticin |
pS13DY | invalid | Lhom + PgpdA + nptIIBB + Rhom | BK, BM ,BQ | pOSCAR | Spectinomycin | Geneticin |
pS13DZ | invalid | Lhom + PgpdA + hphBB + Rhom | BK, BM, BR | pOSCAR | Spectinomycin | Hygromycin |
pAh13EA | invalid | hph + C. heterostrophus Pgpd | BK, BM, BR | pHPVEL1 | Spectinomycin | Hygromycin |
pS13EB | invalid | pOSCAR backbone for transformation into agrobacterium | BK, BM, BR | pOSCAR | Spectinomycin | Hygromycin |