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| - | <li><a href="/Team:SJTU-BioX-Shanghai">Home</a></li>
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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project">Overview</a>
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| - | <li>
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| - | < li><a href="/ Team:SJTU-BioX-Shanghai/ Parts"> Parts</a ></li> | + | <font size=5><stong>?</strong></font> How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ CRISPRi-on"> Prospect</a> | + | <br> |
| - | <ul>
| + | <font size=5><stong>?</strong></font> And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ CRISPRi-on"> CRISPRi-on</a ></li> | + | <br><br> |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ Smaller_light_sensors"> Samaller Light Sensor</a ></li> | + | For decades, these questions have haunted “bioengineers”(especially synthetic biologists), who would like to have certain metabolites produced in living cells. |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ Absolutely_automatic"> Absolutely Automatic</a></ li> | + | <br> |
| - | </ul>
| + | The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome. |
| - | </li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Team</a>
| + | <br> |
| - | <ul>
| + | So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. We offer our product, the METABOLIC GEAR BOX, as a versatile BLACK BOX that can be conveniently applied in the optimization of whatever pathways. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/Applications/ Versatile#Tutorial/General_Protocol"> Tutorial/General Protocol (How to Use the Box)</a> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Team/members">Members</a></li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Attributions</a></li>
| + | <br> |
| - | </ul>
| + | Inside the BOX, we integrate Luminous System, Light Sensors and CRISPRi. Luminous system and light sensors are used to address the problem of multiple gene regulation, while CRISPRi addresses the problem of genomic regulation. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Motivation"> Problems</a> and <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Inside_the_BOX"> Our Solutions</a> |
| - | </li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a>
| + | </div> |
| - | <ul>
| + | </section> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Protocol</a>
| + | <section class="row-padded"><div class="grid-container"><div class="grid-row row-4 "><div class="grid-unit col-3"><div id="pivot-1" class="pivot " bi:type="pivot"><h2 class="heading" bi:titleflag="t1" bi:title="t1">DIY！ ( *・ω・)✄</h2><div class="grid-row row-3 features"><div class="grid-unit"> |
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| - | </li>
| + | </div> |
| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human"> Human Practice</a> | + | <div class=" highlight-text"> |
| - | < ul>< li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human"> BioCraft</a> </li> | + | |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/CC"> Communications on Campus</a></ li> | + | |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human/RSB">Reserch on Synthetic Biology</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ safety"> Safety< /a></li> | + | |
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| - | <div id="abstract">
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| - | <div id="abstract-title"><p><strong style="color:rgb(255,102,102); font-size:60px;">A</strong>bstract</p></div>
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| - | <p>How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome.</p>
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| - | <p>So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating <strong>CRISPRi</strong>, the newly developed expression interference tool, with three <strong>light sensors</strong> (namely <font color=red>red light sensor</font>, <font color=green>green light sensor</font> and <font color=blue>blue light sensor</font>), we expect to provide a <strong>versatile</strong> platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to <strong>change the target, simply change the small guide RNA</strong> (sgRNA)!</p>
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| - | <p>In addition, with the <strong>luminous device and accompanying software</strong>, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct <strong>flux balance analysis (FBA)</strong>, giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into <strong>intensity of lights</strong> that are finally to be exerted on cell cultures. It is -- just convenient! :)</p>
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">
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| - | <p>Luminous System provide us a fascinating tool to adjust expression </p>
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| - | <div id="achievements-title"><p><strong style="color:rgb(102,102,255); font-size:60px;">A</strong>chievements</p></div>
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| - | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> Redefinition of scaffold in Synthetic Biology by recruiting <i>E.coli’</i>s inner membrane as a natural two-dimensional scaffold. </p>
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| - | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> The system we built:</font></strong> 6 membrane proteins orderly organized on the inner membrane of <i>E.coli</i>, the efficiency of which has been proved by fluorescence complementation assay and biosynthesis experiment.</p>
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| - | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> The device we created – Membrane Accelerator:</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p>
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| - | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> device we created – Membrane Rudder:</font></strong> A universal tool used to dynamically and artificially control biochemical reactions in <i>E.coli</i>; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched. </p>
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| - | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> New direction we proposed:</font></strong> The application of scaffold system in accelerating biodegradation pathway using our Membrane Accelerator.</p>
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| - | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> 42 well-characterized parts that could either be used directly or serve as a universal tool readily for potential scientific or engineering use.</p>
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| - | <p><strong><font face="Wingdings " size="4">ü</font><font face="Cambria, serif " size="4"> A club we established – BioCraft:</font></strong> The headquarter of our human practice programs, having come a long way in propagandizing Synthetic Biology and iGEM. Warmly-received activities have been held in and outside the campus. Several celebrities in different fields have shown support for us, laying a cornerstone for our future development.</p>
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