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| - | <ul >
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| - | <li><a href="/Team:SJTU-BioX-Shanghai">Home</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Project">Project</a>
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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project">Overview</a>
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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">Luminous System</a>
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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/ Regulator/Design_criteria"> Regulator-CRISPRi</a > | + | <div class="ws_bullets"><div> |
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| - | <ul>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling/Light_sensor_characteristic">Light sensor characteristic</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling/Report_gene_prediction">Report gene prediction</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Modeling/ Metabolize_optimization"> Metabolize optimization</a></li> | + | <script src="/Team:SJTU-BioX-Shanghai/13SJTU_wowscript.js?action=raw&ctype=text/javascript"></script> |
| - | </ul>
| + | <section class="row-padded-top row-grey"> |
| - | <li>
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| - | < li><a href="/ Team:SJTU-BioX-Shanghai/ Parts"> Parts</a ></li> | + | <font size=5><stong>?</strong></font> How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ CRISPRi-on"> Prospect</a> | + | <br> |
| - | <ul>
| + | <font size=5><stong>?</strong></font> And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ CRISPRi-on"> CRISPRi-on</a ></li> | + | <br><br> |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ Smaller_light_sensors"> Samaller Light Sensor</a ></li> | + | For decades, these questions have haunted “bioengineers”(especially synthetic biologists), who would like to have certain metabolites produced in living cells. |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect/ Absolutely_automatic"> Absolutely Automatic</a></ li> | + | <br> |
| - | </ul>
| + | The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome. |
| - | </li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Team</a>
| + | <br> |
| - | <ul>
| + | So this year, our team, SJTU-BioX-Shanghai , is dedicated to solve the problem. We offer our product, the METABOLIC GEAR BOX, as a versatile BLACK BOX that can be conveniently applied in the optimization of whatever pathways. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Applications/ Versatile#Tutorial/General_Protocol"> Tutorial/General Protocol (How to Use the Box)</a> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Team/members">Members</a></li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Team">Attributions</a></li>
| + | <br> |
| - | </ul>
| + | Inside the BOX, we integrate Luminous System, Light Sensors and CRISPRi. Luminous system and light sensors are used to address the problem of multiple gene regulation, while CRISPRi addresses the problem of genomic regulation. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Motivation"> Problems</a> and <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Inside_the_BOX"> Our Solutions</a> |
| - | </li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a>
| + | </div> |
| - | <ul>
| + | </section> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Protocol</a>
| + | <section class="row-padded"><div class="grid-container"><div class="grid-row row-4 "><div class="grid-unit col-3"><div id="pivot-1" class="pivot " bi:type="pivot"><h2 class="heading" bi:titleflag="t1" bi:title="t1">DIY！ ( *・ω・)✄</h2><div class="grid-row row-3 features"><div class="grid-unit"> |
| - | </li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/Lab_log/July">Lablog</a>
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| - | </li>
| + | <div class="highlight-img"> |
| - | </ul>
| + | <img bi:parenttitle="t1" src="/wiki/images/e/e7/13sjtudiy1.JPG" alt="How to make the box?" bi:mimiclink="true" bi:type="img" /> |
| - | </li>
| + | </div> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">Human Practice</a>
| + | <div class=" highlight-text"> |
| - | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">BioCraft</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/ CC"> Communications on Campus</a>< /li> | + | |
| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/RSB"> Reserch on Synthetic Biology</a> </li> | + | |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/human/ Cooperation"> Cooperation</a>< /li> | + | |
| - | </ul>
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| - | </li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Safety">Safety</a>
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| - | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Safety">Safety Form</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Safety/Security">Security</a></li>
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| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Safety/ Environment"> Environment< /a></li> | + | |
| - | </ul></li>
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| | + | <p bi:parenttitle="t1" bi:mimiclink="true" bi:type="desc">How to assemble the box?</p> |
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| - | <tr><!-- abstract -->
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| - | <div id="abstract">
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| - | <div id="abstract-title"><p><strong style="color:rgb(255,102,102); font-size:60px;">A</strong>bstract</p></div>
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| - | <p>How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome.</p>
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| - | <p>So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating <strong>CRISPRi</strong>, the newly developed expression interference tool, with three <strong>light sensors</strong> (namely <font color=red>red light sensor</font>, <font color=green>green light sensor</font> and <font color=blue>blue light sensor</font>), we expect to provide a <strong>versatile</strong> platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to <strong>change the target, simply change the small guide RNA</strong> (sgRNA)!</p>
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| - | <p>In addition, with the <strong>luminous device and accompanying software</strong>, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct <strong>flux balance analysis (FBA)</strong>, giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into <strong>intensity of lights</strong> that are finally to be exerted on cell cultures. It is -- just convenient! :)</p>
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| - | <div id="entry-cover" style="background-image:url(/wiki/images/0/02/Luminous_System.png);">
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">
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| - | <p>Luminous System provide us a fascinating tool to adjust expression on genomic level.</p><p>Independent luminous sources work through a unbelievable project</p>
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| - | <div id="achievements-title"><p><strong style="color:rgb(102,102,255); font-size:60px;">A</strong>chievements</p></div>
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| - | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> The quantitative regulation of gene expression in the level of genome. </p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The system we built:</font></strong> Assure the effectiveness of the novel system CRISPRi , integration between CRISPRi system and several sensors.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The device we created :</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> device we created – Membrane Rudder:</font></strong> A universal tool used to dynamically and artificially control biochemical reactions in <i>E.coli</i>; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched. </p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The experiment we did:</font></strong> having a great summer filled with experiments, and planning to have fun at the Asian Jamboree!.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> .</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Human practise:</font></strong> involvement in an official academic elite cultivation plan supported by national education ministry. Cooperation with corporation to try applying our project into industry. A survey associated with subject cross and a detailed relevant analysis of this survey.</p>
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