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| - | <li><a href="/Team:SJTU-BioX-Shanghai">Home</a></li>
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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project">Overview</a>
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| - | <li ><a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">Luminous System</a>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Modeling/Light_sensor_characteristic">Light sensor characteristic</a></li>
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| - | <li>
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| - | < li><a href="/ Team:SJTU-BioX-Shanghai/ Parts"> Parts</a ></li> | + | <font size=5><stong>?</strong></font> How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Prospect"> Prospect</a> | + | <br> |
| - | | + | <font size=5><stong>?</strong></font> And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? |
| - | </li>
| + | <br><br> |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Team"> Team</a> | + | For decades, these questions have haunted “bioengineers”(especially synthetic biologists), who would like to have certain metabolites produced in living cells. |
| - | <ul>
| + | <br> |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Team/ members"> Members & Attribtutions</a ></li> | + | The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome. |
| - | <li><a href="/ Team:SJTU-BioX-Shanghai/ Team"> Attributions</a></ li> | + | <br> |
| - | </ul>
| + | <br> |
| - | </li>
| + | So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. We offer our product, the METABOLIC GEAR BOX, as a versatile BLACK BOX that can be conveniently applied in the optimization of whatever pathways. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Applications/ Versatile#Tutorial/General_Protocol"> Tutorial/General Protocol (How to Use the Box)</a> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Notebook</a>
| + | <br> |
| - | <ul>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/protocol">Protocol</a>
| + | Inside the BOX, we integrate Luminous System, Light Sensors and CRISPRi. Luminous system and light sensors are used to address the problem of multiple gene regulation, while CRISPRi addresses the problem of genomic regulation. See <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Motivation"> Problems</a> and <a href=" http://igem.bio-x.cn/Team:SJTU-BioX-Shanghai/ Project/ Overview#Inside_the_BOX"> Our Solutions</a> |
| - | </li>
| + | <br> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Notebook/Lab_log/July">Lablog</a>
| + | </div> |
| - | </li>
| + | </section> |
| - | </ul>
| + | <section class="row-padded"><div class="grid-container"><div class="grid-row row-4 "><div class="grid-unit col-3"><div id="pivot-1" class="pivot " bi:type="pivot"><h2 class="heading" bi:titleflag="t1" bi:title="t1">DIY！ ( *・ω・)✄</h2><div class="grid-row row-3 features"><div class="grid-unit"> |
| - | </li>
| + | <div bi:type="highlight" class="hpHighlight "> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">Human Practice</a>
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| - | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Consideration/human">BioCraft</a></li>
| + | <div class="highlight-img"> |
| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/ CC"> Communications on Campus</a>< /li> | + | <img bi:parenttitle="t1" src="/wiki/images/e/e7/13sjtudiy1.JPG" alt="How to make the box?" bi:mimiclink="true" bi:type="img" /> |
| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/ human/RSB"> Reserch on Synthetic Biology</a> </li> | + | </div> |
| - | <li><a href="/Team:SJTU-BioX-Shanghai/ Consideration/human/ Cooperation"> Cooperation</a>< /li> | + | <div class=" highlight-text"> |
| - | </ul>
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| - | </li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Safety">Safety</a>
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| - | <ul><li><a href="/Team:SJTU-BioX-Shanghai/Safety">Safety Form</a></li>
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| - | <li><a href="/Team:SJTU-BioX-Shanghai/Safety/Security">Security</a></li>
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| - | < li><a href="/Team:SJTU-BioX-Shanghai/ Safety/ Environment"> Environment< /a></li> | + | |
| - | </ul></li>
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| - | <div id="abstract">
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| - | <div id="abstract-title"><p><strong style="color:rgb(255,102,102); font-size:60px;">A</strong>bstract</p></div>
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| - | <div id="abstract-content">
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| - | <p>How to regulate an <strong>entire metabolic pathway</strong> <font style="font-style:italic;">in vivo</font>, delicately, <strong>accurately and conveniently</strong>, simultaneously controlling the expression of <strong>several</strong> genes? And how to <strong>optimize metabolic fluxes so as to maximize desired products</strong>? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the <strong>genome</strong>, or have been implemented into the genome.</p>
| + | |
| - | <p>So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating <strong>CRISPRi</strong>, the newly developed expression interference tool, with three <strong>light sensors</strong> (namely <font color=red>red light sensor</font>, <font color=green>green light sensor</font> and <font color=blue>blue light sensor</font>), we expect to provide a <strong>versatile</strong> platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to <strong>change the target, simply change the small guide RNA</strong> (sgRNA)!</p>
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| - | <p>In addition, with the <strong>luminous device and accompanying software</strong>, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct <strong>flux balance analysis (FBA)</strong>, giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into <strong>intensity of lights</strong> that are finally to be exerted on cell cultures. It is -- just convenient! :)</p>
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| - | <div id="entry-cover" style="background-image:url(/wiki/images/0/02/Luminous_System.png);">
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Luminous_device/Design">
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| - | <p>Luminous System provides us a fascinating tool to adjust expression on genomic level.</p><p>Independent luminous sources work through an unbelievable project</p>
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Light_sensor">
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| - | <p>这里写</p></div></a>
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| - | <a href="/Team:SJTU-BioX-Shanghai/Project/Regulator">
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| - | <p>这里写</p></div></a
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| - | <div id="achievements-title"><p><strong style="color:rgb(102,102,255); font-size:60px;">A</strong>chievements</p></div>
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| - | <p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> The quantitative regulation of gene expression in the level of genome. </p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The system we built:</font></strong> Assure the effectiveness of the novel system CRISPRi , integration between CRISPRi system and several sensors.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The device we created :</font></strong> An electronic device constructed to measure the parameters of LED.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Helping other teams:</font></strong> Sending parts to Zhejiang University and the Chinese University of Hong Kong, helping improving the modeling of the projects of NTU-Taida and NTU Taiwan in the 2013 conference, help students from Shandong University to construct their own team. </p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The experiment we did:</font></strong> Having a great summer filled with experiments, and planning to have fun at the Asian Jamboree!.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> We make components of popular CRISPRi into parts and improve several parts by inserting them into constitutive expression operon.</p>
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| - | <p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Human practise:</font></strong> Involvement in an official academic elite cultivation plan supported by national education ministry. Cooperation with corporation to try applying our project into industry. A survey associated with subject cross and a detailed relevant analysis of this survey.</p>
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