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Team:Penn/MethylaseOverview
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| - | <header><h1><b><center>< | + | <header><h1><b><center>Site-Specific Methylases</center></b></h1></header> |
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| + | For a detailed, graphical explanation of the MaGellin work flow, please download the <a href="https://static.igem.org/mediawiki/2013/e/e5/Spec_Sheet.pdf">MaGellin Workflow Specifications Sheet</a>, which includes all of the steps in the MaGellin workflow. | ||
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| + | After validating the MaGellin assay with non-specific methylases, we were ready to test site-specific methylases. Our MaGellin assay is ideal for high-throughput construction and testing of these enzymes. Site-specific methylases are fusion proteins: a DNA binding domain linked to a methylase by a serine glycine chain. They can direct DNA methylation to specific sequences, likely promoter regions for use as a transcriptional silencer. We used the prokaryotic methylase M.SssI for all of our studies (BBa_K1128000). | ||
| + | <p>We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize a novel site-specific methylase using a TALE DNA binding domain. | ||
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| + | <center><a href="https://2013.igem.org/Team:Penn/Software">←Previous</a> <a href="https://2013.igem.org/Team:Penn/MethylaseCharacterization">Next→</a></center> | ||
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Latest revision as of 03:27, 29 October 2013
Site-Specific Methylases
We wanted to first recapitulate published results with a zinc finger binding domain, and then characterize a novel site-specific methylase using a TALE DNA binding domain.
