Team:Alberta/FAQ

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   <body>
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     <div class="titlebar">
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      <h3>The Littlest Mapmaker</h3>
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<br>
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      <h4>"Exploration into the world of <a href="#">DNA Computing</a>"<br/>
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<img src="/wiki/images/4/41/Ab_mapmen_title.png" width="600" height="95"></img><img src="/wiki/images/a/a5/2013-igem-logo.png" width="198" height="95"></img>
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        Team Alberta: University of Alberta</h4>
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      <a href="http://www.ualberta.ca" class="ualberta-logo"><img src="/wiki/images/b/b3/Ualberta-logo.png" alt="University of Alberta"></img></a>
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      <a href="https://2013.igem.org" class="igem-logo"><img src="/wiki/images/a/ad/IGem-logo.png" alt="iGem Main Page"></img></a>
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     </div>
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         <li><a id="project" href="#">Project</a>
         <li><a id="project" href="#">Project</a>
           <ul>
           <ul>
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             <li><a href="/Team:Alberta/Project">Overview</a></li>
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             <li><a href="/Team:Alberta/Background">Background</a></li>
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             <li><a href="/Team:Alberta/Methodology">Methodology</a></li>
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             <li><a href="/Team:Alberta/Overview">Overview</a></li>
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             <li><a href="/Team:Alberta/Applications">Applications</a></li>
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             <li><a href="/Team:Alberta/Results">Results</a></li>
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          </ul>
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            <li><a href="/Team:Alberta/Protocols">Protocols</a></li>
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        </li>
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             <li><a href="/Team:Alberta/Parts">Submitted Parts</a></li>
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        <li><a id="notebook" href="#">Notebook</a>
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             <li><a href="/Team:Alberta/Accomplishments">Accomplishments</a></li>
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          <ul>
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             <li><a href="/Team:Alberta/Notebook">Timeline</a></li>
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             <li><a href="/Team:Alberta/Parts">BioBricks</a></li>
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           </ul>
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             <li><a href="/Team:Alberta/Team">Roster</a></li>
             <li><a href="/Team:Alberta/Team">Roster</a></li>
             <li><a href="https://igem.org/Team.cgi?year=2013&team_name=Alberta">Official Profile</a></li>
             <li><a href="https://igem.org/Team.cgi?year=2013&team_name=Alberta">Official Profile</a></li>
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             <li><a href="/Team:Alberta/Attributions">Sponsors</a></li>
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             <li><a href="/Team:Alberta/Sponsors">Sponsors</a></li>
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            <li><a href="/Team:Alberta/Attributions">Attributions</a></li>
           </ul>
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           </ul>
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         <li><i class="nav-icon"></i><a id="safety" href="#" class="active">Safety</a>
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         <li><i class="nav-icon"></i><a id="safety" href="/Team:Alberta/MainSafety" class="active">Safety</a>
           <ul>
           <ul>
             <li><a href="/Team:Alberta/SafetyForm">Safety Form</a></li>
             <li><a href="/Team:Alberta/SafetyForm">Safety Form</a></li>
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       </ul>
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       <div class="sidebar">
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          <span class="saying">Welcome to the Team Alberta Wiki!
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          <h5>Other Sections</h5>
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             <div id="box" style="width: 450px; padding: 5px; border: 3px solid #000; background-color: #000000;">
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            <a href="/Team:Alberta/SafetyForm"><p>Safety Form</p></a>
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             <a href="/Team:Alberta/FAQ" class="active"><p>FAQ</p></a>
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              For visitors: this site is currently under construction. Please contact our Student Liason, Dawson at                  daocun@ualberta.ca, for more information on our current project and how to support us!
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             <a href="/Team:Alberta/Resources"><p>Resources</p></a>
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            </div>
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            </div>
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          </span>
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         </div>
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       <div class="main">
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         <p class="content-title">Community</p>
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         <p class="content-title"><img src="/wiki/images/thumb/b/b0/Public_members.png/463px-Public_members.png " height="120"></img>  FAQ</p>
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       <div class="block">
       <div class="block">
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         <p><b>Get an inside look at the project by following us on social media.<b></p>
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         <h3>Doesn’t <i>E. coli</i> cause food poisoning? If <i>E. coli</i> is so dangerous, why are they being used in labs?</h3>
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         <ul>
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         <p style="float:left;margin-left:85px;"><a href="https://twitter.com/iGEMAlberta"><img src="/wiki/images/thumb/e/e8/Twit.png/120px-Twit.png" style="height:150px;" alt="Twitter"></img></a>
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          <li>Normal <i>E. coli</i> colonize the lower intestine of humans, while <b>producing Vitamin K</b> and
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        <a href="https://www.facebook.com/pages/University-of-Alberta-iGEM-Team/117860431757664"><img src="/wiki/images/2/2b/Facebook-icon-256x256.png" style="height:150px;" alt="Facebook"></img></a></a>
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            <b>protecting infection</b> from other harmful bacteria.</li>
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         <a href="http://statigr.am/tag/igemalberta2013"><img src="/wiki/images/9/93/Instagram-icon-256x256.png" style="height:150px;" alt="Instagram"></img></a></p>
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          <li>Only some strains of <i>E. coli</i> cause food poisoning, but our group is using a <b>harmless,
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            nonpathogenic strain</b>. This train is engineered to contain mutations that would not allow the <i>E.  
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            coli</i> to survive outside the lab without particular nutrients.</li>
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          <li><i>E. coli</i> provides a well-studied and well-known <b>model system</b> to test and troubleshoot new
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            trials on.</li>
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        </ul>
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         <p>Reference: <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1728375/">
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          http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1728375/</a></p>
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        <h3>What if plasmids containing <b>antibiotic resistance genes</b> were released into the environment despite
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          precautions?</h3>
 +
        <ul>
 +
          <li>These genes are used in synthetic biology as selection tools.</li>
 +
          <li>There are lower chances of gene uptake by wild bacteria compared to lab-strain bacteria which
 +
            have perforated membranes.</li>
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          <li>In the case that wild bacteria did uptake the antibiotic resistance gene, that gene would
 +
            eventually be lost in the population without exposure to the antibiotic, because it is then a
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            disadvantage for the bacteria to carry the unused plasmids. (Evidence observed in past with the
 +
            decline of specific antibiotic resistance after the human population limited that antibiotic).</li>
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        </ul>
       </div>
       </div>
       </div>
       </div>

Latest revision as of 03:53, 29 October 2013


Other Sections

Safety Form

FAQ

Resources

FAQ

Doesn’t E. coli cause food poisoning? If E. coli is so dangerous, why are they being used in labs?

  • Normal E. coli colonize the lower intestine of humans, while producing Vitamin K and protecting infection from other harmful bacteria.
  • Only some strains of E. coli cause food poisoning, but our group is using a harmless, nonpathogenic strain. This train is engineered to contain mutations that would not allow the E. coli to survive outside the lab without particular nutrients.
  • E. coli provides a well-studied and well-known model system to test and troubleshoot new trials on.

Reference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1728375/

What if plasmids containing antibiotic resistance genes were released into the environment despite precautions?

  • These genes are used in synthetic biology as selection tools.
  • There are lower chances of gene uptake by wild bacteria compared to lab-strain bacteria which have perforated membranes.
  • In the case that wild bacteria did uptake the antibiotic resistance gene, that gene would eventually be lost in the population without exposure to the antibiotic, because it is then a disadvantage for the bacteria to carry the unused plasmids. (Evidence observed in past with the decline of specific antibiotic resistance after the human population limited that antibiotic).

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