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Team:Freiburg
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<img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png"> | <img id="klammer_gross_links" src="https://static.igem.org/mediawiki/2013/e/e0/Freiburg_2013_main2_Rahmen_gross_links.png"> | ||
| - | <div id=" | + | <div id="text1"> |
<div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div> | <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div> | ||
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regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising | regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising | ||
CRISPR/Cas9 system. <br> | CRISPR/Cas9 system. <br> | ||
| - | The regulation is based on the RNA-guided | + | The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. |
| - | Our toolkit comprises not only a standardized | + | Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for |
efficient gene activation or repression. | efficient gene activation or repression. | ||
</div> | </div> | ||
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<ul style="margin-left:160px"> | <ul style="margin-left:160px"> | ||
| - | <li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna | + | <li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna"> |
<img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png"> | <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png"> | ||
<span> Multiple targeting </span> | <span> Multiple targeting </span> | ||
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<div id="text-box3"> | <div id="text-box3"> | ||
<div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a> | <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a> | ||
| - | <p style="position:absolute; top:720px; left:60px" > All about our <br> project | + | <p style="position:absolute; top:720px; left:60px" > All about our <br> project in one <br> slide show! </p> |
<img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1"> | <img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1"> | ||
</div> | </div> | ||
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<div id="text-box6"> | <div id="text-box6"> | ||
| - | + | <div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/News"> News </a></div> | |
| - | + | <p style="padding-top:10px;"> | |
| + | Have a look at our achievements in Lyon & Boston! | ||
| + | </p> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/5/56/Best-foundational-advance-2013-freiburg.png" height="130" style="position:absolute; top:600px; left:980px; z-index:1"> | ||
</div> | </div> | ||
| + | </div> | ||
<img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png"> | <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png"> | ||
</td> | </td> | ||
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Latest revision as of 23:52, 9 April 2014
Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene
regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising
CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression. 
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