Team:Hong Kong HKU

From 2013.igem.org

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<!doctype html>
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{{:Team:Hong_Kong_HKU/templates/header_frontpage}}
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<html>
<html>
<head>
<head>
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<script src="http://ajax.googleapis.com/ajax/libs/jquery/1.10.2/jquery.min.js"></script>
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<script src="http://www.buzz-code.com/file/hku_igem/lean-slider.js"></script>
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<link rel="stylesheet" href="http://www.buzz-code.com/file/hku_igem/lean-slider.css" type="text/css" />
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<meta charset="utf-8">
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<link rel="stylesheet" href="http://www.buzz-code.com/file/hku_igem/sample-styles.css" type="text/css" />
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<title>E. capsi</title>
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<style type="text/css">
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body,td,th {
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font-family: "Gill Sans", "Gill Sans MT", "Myriad Pro", "DejaVu Sans Condensed", Helvetica, Arial, sans-serif;
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font-style: normal;
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}
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body {
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background-image: url("https://static.igem.org/mediawiki/2013/0/02/Hku_background.jpg");
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background-repeat: repeat;
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}
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</style>
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</head>
</head>
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<body>
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<body bgcolor="#000000">
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<div id="divWrapper">
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<div id="wrapper">  
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<div id="divHeader"><img src="https://static.igem.org/mediawiki/2013/c/cb/Hku_logo.jpg" width="960" height="240"/></div>
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    <div id="divlinks">
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    <div id="divNav">
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        <style type="text/css">
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.tab{
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font-family: arial, verdana, san-serif;
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font-size: 14px;
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}
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.asd
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{
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text-decoration: none;
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font-family: arial, verdana, san-serif;
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font-size: 13px;
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color:00CC66;
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}
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/*****remove the list style****/
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<div id="slider">
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#nav {
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    <div class="slide1">
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margin:0;
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        <img src="https://static.igem.org/mediawiki/2013/c/c1/Panel_Welcome_00000_00000.jpg" alt="" />
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padding:0;
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    </div>
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list-style:none;
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    <div class="slide2">
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}
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        <img src="https://static.igem.org/mediawiki/2013/2/2a/Panel_Biobricks_00000_00000.jpg" alt="" />
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    </div>
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    <div class="slide3">
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        <img src="https://static.igem.org/mediawiki/2013/5/58/Panel_Human_Practice_00000_00000.jpg" alt="" />
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    </div>   
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    <div class="slide4">
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        <img src="https://static.igem.org/mediawiki/2013/0/00/Panel_Applications_00000_00000.jpg" alt="" />
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    </div>
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</div>mhvnh h
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    <div id="slider-direction-nav"></div>
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    <div id="slider-control-nav"></div>
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/*****LI display inline *****/
 
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#nav li {
 
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float:left;
 
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display:block;
 
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width:190px;
 
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background:#00661A;
 
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position:relative;
 
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z-index:500;
 
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margin:0 1px;
 
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}
 
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@charset "utf-8";
 
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body, h1, h2, h3, h4, h5, h6, p, div, ul , li{
 
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margin:0;
 
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padding:0;
 
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}
 
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<br>
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<table width="960px" cellpadding="0px">
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<tr>
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<td>
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<p>
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Project" target="_new">
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<img src="https://static.igem.org/mediawiki/2013/2/24/New_button_take_a_glance_00000.jpg" width="236" />
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</a>
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Parts" target="_new">
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<img src="https://static.igem.org/mediawiki/2013/6/68/New_button_biobricks_00000.jpg" width="236"  />
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</a>
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/humanpractice/talk" target="_new">
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<img src="https://static.igem.org/mediawiki/2013/5/56/New_button_Human_Practice_00000.jpg" width="236"  />
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</a>
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/humanpractice/futureapps" target="_new">
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<img src="https://static.igem.org/mediawiki/2013/e/ee/New_button_applications_00000.jpg" width="236" />
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</a>
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</p>
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</td>
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</tr>
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</table>
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<br><br>
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</div>
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<script>
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$(document).ready(function() {
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    $('#slider').leanSlider({
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            directionNav: '#slider-direction-nav',
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            controlNav: '#slider-control-nav'
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        });
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});
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body {
 
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font-family:Segoe, "Segoe UI", "DejaVu Sans", "Trebuchet MS", Verdana, sans-serif;
 
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}
 
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#divWrapper{
 
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width:960px;
 
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margin:0 auto;
 
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}
 
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#divHeader{
 
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width:960px;
 
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margin:0 auto;
 
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text-align:center;
 
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}
 
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#divHeader img{
 
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padding-top:20px;
 
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padding-bottom:5px;
 
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}
 
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#divline {
 
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width:960px;
 
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background:#F63;
 
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height:2px;
 
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}
 
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#divlinks{
 
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width:960px;
 
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background:#666;
 
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}
 
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#divNav{
 
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width:960px;
 
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background:#000;
 
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height:32px;
 
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margin:0 auto;
 
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}
 
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#divAbout {
 
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width:960px;
 
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background:#000;
 
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margin:0 auto;
 
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}
 
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#divContent{
 
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width:960px;
 
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height:1000px
 
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margin:0 auto;
 
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border:1px auto solid #000;
 
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text-align:center;
 
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}
 
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#divContent img{
 
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float:left;
 
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border:1px solid #000;
 
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background #333;
 
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padding:16px;
 
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margin:0.7em;
 
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background:-moz-linear-gradient(top,#666,#333);
 
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background:-webkit-gradient(linear,left,top, left bottom, from (#666), to(#333));
 
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}
 
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/*****parent menu*****/
 
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#nav li a {
 
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display:block;
 
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padding:8px 5px 0 5px;
 
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font-weight:700;
 
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height:23px;
 
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text-decoration:none;
 
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color:#00994D;
 
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text-align:center;
 
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color:#FFCC33;
 
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}
 
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#nav li a:hover
 
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{
 
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color:#FFFFFF;
 
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}
 
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/* style for default selected value */ #nav a.selected {
 
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color:00CC66;
 
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}
 
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/* submenu */ #nav ul
 
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{
 
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position:absolute;
 
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left:0;
 
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display:none;
 
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margin:0 0 0 -1px;
 
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padding:0;
 
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list-style:none;
 
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}
 
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#nav ul li
 
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{
 
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width:190px;
 
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float:left;
 
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border-top:1px solid #fff;
 
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}
 
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/* display block will make the link fill the whole area of LI */ #nav ul a
 
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{
 
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display:block;
 
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height:23px;
 
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padding: 8px 5px;
 
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color:#FFFFF
 
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}
 
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#nav ul a:hover
 
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{
 
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text-decoration:underline;
 
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padding-left:15px;
 
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}
 
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</style>
 
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<script type="text/javascript">
 
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for(var j in a)if(a[j]&&a[j].constructor==Array)jQuery.each(a[j],function(){s.push(encodeURIComponent(j)+"="+encodeURIComponent(this));});else
 
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s.push(encodeURIComponent(j)+"="+encodeURIComponent(jQuery.isFunction(a[j])?a[j]():a[j]));return s.join("&").replace(/%20/g,"+");}});jQuery.fn.extend({show:function(speed,callback){return speed?this.animate({height:"show",width:"show",opacity:"show"},speed,callback):this.filter(":hidden").each(function(){this.style.display=this.oldblock||"";if(jQuery.css(this,"display")=="none"){var elem=jQuery("<"+this.tagName+" />").appendTo("body");this.style.display=elem.css("display");if(this.style.display=="none")this.style.display="block";elem.remove();}}).end();},hide:function(speed,callback){return speed?this.animate({height:"hide",width:"hide",opacity:"hide"},speed,callback):this.filter(":visible").each(function(){this.oldblock=this.oldblock||jQuery.css(this,"display");this.style.display="none";}).end();},_toggle:jQuery.fn.toggle,toggle:function(fn,fn2){return jQuery.isFunction(fn)&&jQuery.isFunction(fn2)?this._toggle.apply(this,arguments):fn?this.animate({height:"toggle",width:"toggle",opacity:"toggle"},fn,fn2):this.each(function(){jQuery(this)[jQuery(this).is(":hidden")?"show":"hide"]();});},slideDown:function(speed,callback){return this.animate({height:"show"},speed,callback);},slideUp:function(speed,callback){return this.animate({height:"hide"},speed,callback);},slideToggle:function(speed,callback){return this.animate({height:"toggle"},speed,callback);},fadeIn:function(speed,callback){return this.animate({opacity:"show"},speed,callback);},fadeOut:function(speed,callback){return this.animate({opacity:"hide"},speed,callback);},fadeTo:function(speed,to,callback){return this.animate({opacity:to},speed,callback);},animate:function(prop,speed,easing,callback){var optall=jQuery.speed(speed,easing,callback);return this[optall.queue===false?"each":"queue"](function(){if(this.nodeType!=1)return false;var opt=jQuery.extend({},optall),p,hidden=jQuery(this).is(":hidden"),self=this;for(p in prop){if(prop[p]=="hide"&&hidden||prop[p]=="show"&&!hidden)return opt.complete.call(this);if(p=="height"||p=="width"){opt.display=jQuery.css(this,"display");opt.overflow=this.style.overflow;}}if(opt.overflow!=null)this.style.overflow="hidden";opt.curAnim=jQuery.extend({},prop);jQuery.each(prop,function(name,val){var e=new jQuery.fx(self,opt,name);if(/toggle|show|hide/.test(val))e[val=="toggle"?hidden?"show":"hide":val](prop);else{var parts=val.toString().match(/^([+-]=)?([\d+-.]+)(.*)$/),start=e.cur(true)||0;if(parts){var end=parseFloat(parts[2]),unit=parts[3]||"px";if(unit!="px"){self.style[name]=(end||1)+unit;start=((end||1)/e.cur(true))*start;self.style[name]=start+unit;}if(parts[1])end=((parts[1]=="-="?-1:1)*end)+start;e.custom(start,end,unit);}else
 
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e.custom(start,val,"");}});return true;});},queue:function(type,fn){if(jQuery.isFunction(type)||(type&&type.constructor==Array)){fn=type;type="fx";}if(!type||(typeof type=="string"&&!fn))return queue(this[0],type);return this.each(function(){if(fn.constructor==Array)queue(this,type,fn);else{queue(this,type).push(fn);if(queue(this,type).length==1)fn.call(this);}});},stop:function(clearQueue,gotoEnd){var timers=jQuery.timers;if(clearQueue)this.queue([]);this.each(function(){for(var i=timers.length-1;i>=0;i--)if(timers[i].elem==this){if(gotoEnd)timers[i](true);timers.splice(i,1);}});if(!gotoEnd)this.dequeue();return this;}});var queue=function(elem,type,array){if(elem){type=type||"fx";var q=jQuery.data(elem,type+"queue");if(!q||array)q=jQuery.data(elem,type+"queue",jQuery.makeArray(array));}return q;};jQuery.fn.dequeue=function(type){type=type||"fx";return this.each(function(){var q=queue(this,type);q.shift();if(q.length)q[0].call(this);});};jQuery.extend({speed:function(speed,easing,fn){var opt=speed&&speed.constructor==Object?speed:{complete:fn||!fn&&easing||jQuery.isFunction(speed)&&speed,duration:speed,easing:fn&&easing||easing&&easing.constructor!=Function&&easing};opt.duration=(opt.duration&&opt.duration.constructor==Number?opt.duration:jQuery.fx.speeds[opt.duration])||jQuery.fx.speeds.def;opt.old=opt.complete;opt.complete=function(){if(opt.queue!==false)jQuery(this).dequeue();if(jQuery.isFunction(opt.old))opt.old.call(this);};return opt;},easing:{linear:function(p,n,firstNum,diff){return firstNum+diff*p;},swing:function(p,n,firstNum,diff){return((-Math.cos(p*Math.PI)/2)+0.5)*diff+firstNum;}},timers:[],timerId:null,fx:function(elem,options,prop){this.options=options;this.elem=elem;this.prop=prop;if(!options.orig)options.orig={};}});jQuery.fx.prototype={update:function(){if(this.options.step)this.options.step.call(this.elem,this.now,this);(jQuery.fx.step[this.prop]||jQuery.fx.step._default)(this);if(this.prop=="height"||this.prop=="width")this.elem.style.display="block";},cur:function(force){if(this.elem[this.prop]!=null&&this.elem.style[this.prop]==null)return this.elem[this.prop];var r=parseFloat(jQuery.css(this.elem,this.prop,force));return r&&r>-10000?r:parseFloat(jQuery.curCSS(this.elem,this.prop))||0;},custom:function(from,to,unit){this.startTime=now();this.start=from;this.end=to;this.unit=unit||this.unit||"px";this.now=this.start;this.pos=this.state=0;this.update();var self=this;function t(gotoEnd){return self.step(gotoEnd);}t.elem=this.elem;jQuery.timers.push(t);if(jQuery.timerId==null){jQuery.timerId=setInterval(function(){var timers=jQuery.timers;for(var i=0;i<timers.length;i++)if(!timers[i]())timers.splice(i--,1);if(!timers.length){clearInterval(jQuery.timerId);jQuery.timerId=null;}},13);}},show:function(){this.options.orig[this.prop]=jQuery.attr(this.elem.style,this.prop);this.options.show=true;this.custom(0,this.cur());if(this.prop=="width"||this.prop=="height")this.elem.style[this.prop]="1px";jQuery(this.elem).show();},hide:function(){this.options.orig[this.prop]=jQuery.attr(this.elem.style,this.prop);this.options.hide=true;this.custom(this.cur(),0);},step:function(gotoEnd){var t=now();if(gotoEnd||t>this.options.duration+this.startTime){this.now=this.end;this.pos=this.state=1;this.update();this.options.curAnim[this.prop]=true;var done=true;for(var i in this.options.curAnim)if(this.options.curAnim[i]!==true)done=false;if(done){if(this.options.display!=null){this.elem.style.overflow=this.options.overflow;this.elem.style.display=this.options.display;if(jQuery.css(this.elem,"display")=="none")this.elem.style.display="block";}if(this.options.hide)this.elem.style.display="none";if(this.options.hide||this.options.show)for(var p in this.options.curAnim)jQuery.attr(this.elem.style,p,this.options.orig[p]);}if(done)this.options.complete.call(this.elem);return false;}else{var n=t-this.startTime;this.state=n/this.options.duration;this.pos=jQuery.easing[this.options.easing||(jQuery.easing.swing?"swing":"linear")](this.state,n,0,1,this.options.duration);this.now=this.start+((this.end-this.start)*this.pos);this.update();}return 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<li> <a href='#'>E. Capsid</a>
 
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<ul>
 
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<li style='background-color:#000;'><a href=>At a Glance</a></li>
 
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<li style='background-color:#454141;'><a href=>Surface Decoration</a></li>
 
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By localizing the PPK enzyme into the MCP, the MCP will form a sink for Pi in the system which after normal metabolic uptake by the bacteria is localized to the MCP where it is converted into polyphosphate. As the polyphosphate is too large to pass out of the pores of the MCP, it accumulates in itm preventing phosphate return to the system/ environment.
 
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We have two suitable strains for ppk1 gene (Tanneralla forsythia ATCC 43037. 2.Kingella oralis ATCC 51147) in Rory’s lab. We will clone both native genes and signal-fused gene to compare their enzyme activities. Also, the signal-fused ppk1 will be co-expressed with native MCP and tagged MCP (developed from Surface team) to test the localization of enzyme and Pi clearing efficiency.</p>
 
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Latest revision as of 13:08, 11 October 2014

mhvnh h



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