Team:Hong Kong HKU

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      <meta name="title" content="HKU iGEM 2013" />
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      <meta name="description" content="HKU's IGEM Team. We aim to isolate phosphate using a labelled microcompartment.">
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<p style="color: #000; font-size: 12px; font-family: Baskerville, 'Palatino Linotype', Palatino, 'Century Schoolbook L', 'Times New Roman', serif; text-align: left;">
 
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Project Description:
 
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Background:
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Bacterial Microcompartments (MCP) are closed polyhedral shells, diameter of 100-150nm, which is made of thin protein sheets, enclosing enzymes and cofactors for various forms of fermentative metabolism. Salmonella enteric enthanolamine ultilization (Eut) MCP is one example.
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5 genes (Eut S, M, N, L, K) encodes thousands of copies of shell proteins to form a heterogenous MCP shell and empty recombinant Eut Shell has been successfully expressed in E.coli.  
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In addition, a localization signal (N terminal of EutC19) has been identified and signal fused enzymes/ proteins could be targeted to the cavity of the MCP.
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Project" target="_new">
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<img src="https://static.igem.org/mediawiki/2013/2/24/New_button_take_a_glance_00000.jpg" width="236" />
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Inspired by these studies, we think this Eut MCP can become a versatile tool if we can:
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<br>(a) Modify its exterior surface to enable surface display
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/Parts" target="_new">
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<br>(b) Localize special enzyme into the MCP to enable specific metabolism
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<br>(c) Store useful or harmful molecules into the MCP
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<a href="https://2013.igem.org/Team:Hong_Kong_HKU/humanpractice/talk" target="_new">
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In this iGEM project, using Eut Microcompartment, we aim to show:
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<br>(1) MCP shell surface can be used to display specific peptides, without disrupting the MCP structure, to enable cell-specific targeting for drug delivery system, easy purification etc. We will display Flag and His tag as a proof of concept.
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<br>(2) Localize polyphosphate kinase (ppk1) into the native/ engineered MCP to polymerize inorganic phosphate and accumulate them in MCP, trying to treat phosphate pollution in waste water.
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We will divide into 2 parallel sub-teams, specifically working on surface decoration and ppk enzyme.
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Latest revision as of 13:08, 11 October 2014

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