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Team:Groningen/Labwork/22 July 2013
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(Difference between revisions)
(Created page with "'''Mirjam''' <br/>Made a PCR for silk 2 and 3 (strepF-R and F-strepR)in triplo <br/>- 28 ul MQ <br/>- 1.5 ul DMSO <br/>- 10 ul GC buffer <br/>- 1 ul of each dNTP <br/>- 2.5 ul p...") |
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<br/>- 0.5 ul phusion | <br/>- 0.5 ul phusion | ||
<br/>- 1 ul genomic DNA (BBa_...016) | <br/>- 1 ul genomic DNA (BBa_...016) | ||
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| + | Run a gel with the ligation product of silk and the PCR afterwards. Figured out why the ligation did not work. | ||
| + | |||
| + | Made a new restriction digest for silk without strep tag. | ||
Revision as of 09:01, 22 July 2013
Mirjam
Made a PCR for silk 2 and 3 (strepF-R and F-strepR)in triplo
- 28 ul MQ
- 1.5 ul DMSO
- 10 ul GC buffer
- 1 ul of each dNTP
- 2.5 ul primer F
- 2.5 ul primer R
- 0.5 ul phusion
- 1 ul genomic DNA (BBa_...016)
Run a gel with the ligation product of silk and the PCR afterwards. Figured out why the ligation did not work.
Made a new restriction digest for silk without strep tag.
