Team:Groningen/Labwork/23 July 2013

From 2013.igem.org

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'''Sander'''
'''Sander'''
<br/>Made a restriction digestion for silk strepF-R and silk F-strepR.
<br/>Made a restriction digestion for silk strepF-R and silk F-strepR.
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'''Inne'''
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<br> ''E. coli'' with the BBa_818000 backbone that had to grow overnight grew.
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<table border = "1">
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<tr>
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<td>Sample</td>
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<td>Composition</td>
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<td>BBa_k818000 from</td>
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<td>growth</td>
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</tr>
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<tr>
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<td>1</td>
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<td>4 mL LB, 8 uL Amp</td>
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<td>iGEM 2012</td>
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<td>Yes</td>
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</tr>
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<tr>
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<td>2</td>
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<td>4 mL LB, 8 uL Amp</td>
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<td>iGEM 2012</td>
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<td>Yes</td>
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</tr>
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<tr>
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<td>3</td>
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<td>4 mL, LB 8 uL Amp (negative control)</td>
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<td>iGEM 2012</td>
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<td>No</td>
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</tr>
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<tr>
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<td>4</td>
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<td>4 mL LB, 8 uL Amp, 4 uL IPTG</td>
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<td>iGEM 2012</td>
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<td>Yes</td>
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</tr>
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<tr>
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<td>5</td>
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<td>4 mL LB, 8 uL Amp, 4 uL IPTG</td>
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<td>iGEM 2012</td>
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<td>Yes</td>
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</tr>
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<tr>
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<td>6</td>
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<td>4 mL, 8uL Amp</td>
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<td>iGEM 2013</td>
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<td>Yes</td>
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</tr>
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<tr>
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<td>7</td>
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<td>4 mL, 8 uL Amp, 8uL IPTG</td>
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<td>iGEM 2013</td>
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<td>Yes</td>
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</tr>
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<tr>
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<td>8</td>
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<td>4 mL, 8 uL Amp (negative control)</td>
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<td>iGEM 2013</td>
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<td>No</td>
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</tr>
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</table>

Revision as of 09:09, 23 July 2013

Mirjam
Run a gel with the PCR products of silk without strep + signal sequence.
The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification.

Run a gel with the purified PCR product of silk strepF-R and silk F-strepR.
The products are present in 2/3 samples for each silk type. These are combined and concentrated. Concentrations of 18.1 and 16.1 ng/ul are obtained. So a restriction digestion can be made.

Made a restriction digestion for the signal sequences.


Sander
Made a restriction digestion for silk strepF-R and silk F-strepR.

Inne
E. coli with the BBa_818000 backbone that had to grow overnight grew.

Sample Composition BBa_k818000 from growth
1 4 mL LB, 8 uL Amp iGEM 2012 Yes
2 4 mL LB, 8 uL Amp iGEM 2012 Yes
3 4 mL, LB 8 uL Amp (negative control) iGEM 2012 No
4 4 mL LB, 8 uL Amp, 4 uL IPTG iGEM 2012 Yes
5 4 mL LB, 8 uL Amp, 4 uL IPTG iGEM 2012 Yes
6 4 mL, 8uL Amp iGEM 2013 Yes
7 4 mL, 8 uL Amp, 8uL IPTG iGEM 2013 Yes
8 4 mL, 8 uL Amp (negative control) iGEM 2013 No
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