Team:Groningen/Labwork/4 July 2013

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'''Sander and Inne'''
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<h2>Sander and Inne</h2>
<Br/>For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA  but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
<Br/>For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA  but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
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Ran the same gel again for 22 min on 100 volt to get a better picture
Ran the same gel again for 22 min on 100 volt to get a better picture
<br />(picture was take about an hour and a half after run finished because of our meeting)
<br />(picture was take about an hour and a half after run finished because of our meeting)
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Revision as of 11:57, 24 July 2013

Sander and Inne


For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever Ran a gel with the 8 PCR products and the purified promoter from yesterday (as a check).
Well 1 2 3 4 5 6 7 8 9 10
promoter Marker 1 1. 2 2. 3 3. 4 4.
1. = duplo of sample 1 Ran the same gel again for 22 min on 100 volt to get a better picture
(picture was take about an hour and a half after run finished because of our meeting)

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