Revision as of 13:12, 24 July 2013

208

Henrike, Julia, Gosia, Krystian

We perform USER reaction, samples are as follows:

Reaction is performed in the same way as on 18-07-2013 with increased amount of insert (up to 14 uL due to low DNA concentration).

From last week transformants with AMO and HAO in pZA21 we performed plasmid isolation.

We perform restriction analysis with EcoRI. Expected fragments are as follows:

Navigate to the Previous or the Next Entry

@@ Line 13: / Line 13: @@
 ===USER reaction and transformation ===
-We perform USER reaction in order to create construct of plasmid pZA21 and AMO and other construct - pZA21 and HAO. We add also negative control with water instead of insert.
+We perform USER reaction, samples are as follows:
-Reaction is performed according to our protocol with increased amount of insert.
+* plasmid pZA21 and AMO
+* plasmid pZA21 and HAO
+* negative control with water instead of insert
+Reaction is performed in the same way as on [[Team:DTU-Denmark/Notebook/18_July_2013 |18-07-2013]] with increased amount of insert (up to 14 uL due to low DNA concentration).
 ===Restriction analysis===
+From last week transformants with AMO and HAO in pZA21 we performed plasmid isolation.
+We perform restriction analysis with EcoRI. Expected fragments are as follows:
+*
 ===Primer diluting===
 ===PCR to extract Nir from Pseudosomonas with new USER primers===