Team:Groningen/protocols/PCR
From 2013.igem.org
(Difference between revisions)
Line 32: | Line 32: | ||
<li>H<sub>2</sub>O</li> | <li>H<sub>2</sub>O</li> | ||
<li>Phusion Polymerase</li> | <li>Phusion Polymerase</li> | ||
+ | <li>DNA template</li> | ||
</ul> | </ul> | ||
Revision as of 08:46, 26 July 2013
PCR
Materials:
- PCR tubes
- F- and R-primer
- DNTPs
- Phusion buffer
- H2O
- Phusion Polymerase
- DNA template
Steps: Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf