Team:Groningen/protocols/PCR

From 2013.igem.org

(Difference between revisions)
Line 51: Line 51:
<tr><td>10mM dNTPs</td>
<tr><td>10mM dNTPs</td>
<td>1</td>
<td>1</td>
-
<td>0.2&#956;M</td>
+
<td>200&#956;M</td>
</tr>
</tr>
<tr><td>F-primer</td>
<tr><td>F-primer</td>

Revision as of 09:08, 26 July 2013

PCR


Materials:
  • PCR tubes
  • F- and R-primer
  • DNTPs
  • Phusion buffer
  • H2O
  • Phusion Polymerase
  • DNA template

Steps:
Component 50μl reaction Final concentration
H2O up to 50μl
5x Phusion Buffer 10 1x
10mM dNTPs 1 200μM
F-primer 0.2μM
R-primer 0.2μM
Template DNA 1μl ~1ng
Phusion Pol. 0.5μl 0.02U/μl

Cycling instructions:
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf