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Team:Groningen/protocols/PCR
From 2013.igem.org
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Revision as of 09:10, 26 July 2013
PCR
Materials:
- PCR tubes
- F- and R-primer
- DNTPs
- Phusion buffer
- H2O
- Phusion Polymerase
- DNA template
Steps:
| Component | 50μl reaction | Final concentration |
| H2O | up to 50μl | |
| 5x Phusion Buffer | 10 | 1x |
| 10mM dNTPs | 1 | 200μM |
| F-primer | 1 | 0.2μM |
| R-primer | 1 | 0.2μM |
| Template DNA | 1μl | ~1ng |
| Phusion Pol. | 0.5μl | 0.02U/μl |
Cycling instructions:
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf
iGEM 2013 Groningen
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