Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

(Difference between revisions)
Line 27: Line 27:
<ul type="square">
<ul type="square">
<li>Power supply</li>
<li>Power supply</li>
-
<li>Agarose gel</li>
+
<li>0.8 or 1.5% agarose gel</li>
-
<li>Tray</li>
+
<li>Gel tray</li>
<li>DNA samples</li>
<li>DNA samples</li>
-
<li>DNA Ladder</li>
+
<li>DNA 1kb GeneRuler of Fermentas</li>
</ul>
</ul>
<h3>Procedure:</h3>
<h3>Procedure:</h3>
All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
-
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb Gene Ruler of Fermentas.
+
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
<br>A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
<br>A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
<br>The gel is run in TBE buffer 1x.
<br>The gel is run in TBE buffer 1x.

Revision as of 17:31, 27 July 2013

Gel electrophoresis

Materials:

  • Power supply
  • 0.8 or 1.5% agarose gel
  • Gel tray
  • DNA samples
  • DNA 1kb GeneRuler of Fermentas

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
A 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
The gel is run in TBE buffer 1x.