User:ChristopherW

From 2013.igem.org

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Revision as of 23:36, 27 July 2013

AutoAnnotator by Team TU-Munich 2013

Enter a BioBrick number below, either starting with "BBa_" or just the alphanumeric code!
Alternatively (e.g. for new BioBricks, which aren't in the Registry yet) you can enter the nucleotide sequence directly.

Enter BioBrick number!

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-<html><table border="1" cellspacing="0" width="100%" !important><tr><td colspan="400" width="40%"><strong>BioBrick: <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_">BBa_</a></strong></td><td colspan="600" width="60%"><strong>Automatically determined parameters using the <a href="https://2013.igem.org/Team:TU-Munich/Results/Software">BioBrick-AutoAnnotator</a> version 1.0</strong></td></tr><tr><td colspan="100" width="10%"><strong>RFC standard:</strong></td><td colspan="600" width="60%">RFC 10 BioBrick</td><td colspan="300" width="30%">ORF from 3 to 23 (excluding stop-codon)</td></tr><tr><td colspan="1000"><strong>Nucleotide sequence:</strong> (underlined part encodes the protein, italic parts were added)<br><span style="font-family:'Courier New', Arial;">&nbsp;AT<u>ATGGGGTTA&nbsp;...&nbsp;GTATACTCC</u>TAGC</span></td></tr><tr><td colspan="1000"><strong>Amino acid sequence:</strong><br><span style="font-family:'Courier New', Arial;">&nbsp;&nbsp;1&nbsp;MGLLVYS<br></span></td></tr><tr><td colspan="1000"><strong>Amino acid composition:</strong></td></tr><tr><td colspan="100" width="10.0%">A (Ala)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">C (Cys)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">H (His)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">M (Met)</td><td colspan=" 99" width=" 9.9%">1 (14.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">T (Thr)</td><td colspan="100" width="10.0%">0 (0.0%)</td></tr><tr><td colspan="100" width="10.0%">R (Arg)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Q (Gln)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">I (Ile)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">F (Phe)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">W (Trp)</td><td colspan="100" width="10.0%">0 (0.0%)</td></tr><tr><td colspan="100" width="10.0%">N (Asn)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">E (Glu)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">L (Leu)</td><td colspan=" 99" width=" 9.9%">2 (28.6%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">P (Pro)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">Y (Tyr)</td><td colspan="100" width="10.0%">1 (14.3%)</td></tr><tr><td colspan="100" width="10.0%">D (Asp)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">G (Gly)</td><td colspan=" 99" width=" 9.9%">1 (14.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">K (Lys)</td><td colspan=" 99" width=" 9.9%">0 (0.0%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">S (Ser)</td><td colspan=" 99" width=" 9.9%">1 (14.3%)</td><td colspan=" 1" width=" 0.1%"></td><td colspan="100" width="10.0%">V (Val)</td><td colspan="100" width="10.0%">1 (14.3%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Amino acid counting:</strong></td><td colspan="200" width="20.0%">Total number:</td><td colspan="100" width="10.0%">7</td><td colspan="200" width="20.0%">Positively charged (Arg + Lys):</td><td colspan="100" width="10.0%">0 (0.0%)</td><td colspan="200" width="20.0%">Negatively charged (Asp + Glu):</td><td colspan="100" width="10.0%">0 (0.0%)</td></tr><tr><td colspan="100" width="10.0%"><strong>Biochemical parameters:</strong></td><td colspan="200" width="20.0%">Molecular mass [Da]:</td><td colspan="100" width="10.0%">782.0</td><td colspan="200" width="20.0%">Theoretical pI:</td><td colspan="100" width="10.0%">5.27</td><td colspan="200" width="20.0%">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td colspan="100" width="10.0%">1490 / 1490<br>(all Cys red./ox.)</td></tr><tr><td colspan="100" width="10.0%"><strong>Estimated half-life [h]:</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">>10</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">>20</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">30</td></tr><tr><td colspan="100" width="10.0%"><strong>Codon usage (CAI):</strong></td><td colspan="200" width="20.0%"><i>E. coli</i>:</td><td colspan="100" width="10.0%">acceptable (0.55)</td><td colspan="200" width="20.0%"><i>S. cervisiae:</i></td><td colspan="100" width="10.0%">good (0.63)</td><td colspan="200" width="20.0%">Mammals:</td><td colspan="100" width="10.0%">acceptable (0.42)</td></tr><tr><td colspan="1000" width="100.0%"> The BioBrick-AutoAnnotator was created by <a href="https://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please read the <a href="https://2013.igem.org/Team:TU-Munich/Results/Software">description</a>.</td></tr></table><br></html>
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