Team:Groningen/protocols/GelElectrophoresis

From 2013.igem.org

(Difference between revisions)
Line 27: Line 27:
<ul type="square">
<ul type="square">
<li>Power supply</li>
<li>Power supply</li>
-
<li>0.8 or 1.5% agarose gel</li>
 
<li>Gel tray</li>
<li>Gel tray</li>
 +
<li>TBE buffer 1x
 +
<li>0.8 or 1.5% agarose gel</li>
<li>2x Loading Dye
<li>2x Loading Dye
<li>DNA samples</li>
<li>DNA samples</li>
Line 35: Line 36:
<p>
<p>
-
<br><h5>Procedure:</h5>
+
<h5>Procedure:</h5>
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
-
<br>- Load the samples on gel
+
<br>- Load the samples on a 0.8% or 1.5% agarose gel
<br>- Load the 1kb GeneRuler of Fermentas on gel
<br>- Load the 1kb GeneRuler of Fermentas on gel
<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
<br>- Run the gel at 90V for 24-45 minutes to separate the bands.
-
<p>All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
 
-
<br>The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
 
-
<br>The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.
 
</div>
</div>

Revision as of 10:39, 28 July 2013

Gel electrophoresis

Materials:
  • Power supply
  • Gel tray
  • TBE buffer 1x
  • 0.8 or 1.5% agarose gel
  • 2x Loading Dye
  • DNA samples
  • DNA 1kb GeneRuler of Fermentas

Procedure:
- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.