Team:Groningen/Labwork/29 July 2013

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Run the samples of the colony PCR of BBa_K823823 combined with the LacI promoter made on 27-07 on gel (order 1-8).
Run the samples of the colony PCR of BBa_K823823 combined with the LacI promoter made on 27-07 on gel (order 1-8).
-
<br>Run the purified PCR product of spec on gel.
+
<br>There are no bands around 1500 bp.
-
<br>Run the PCR products of silk 2 on gel to do gel purification.
+
 +
<p>Run the purified PCR product of spec on gel.
 +
<br>The purified spec shows a nice band.
 +
<p>Run the PCR products of silk 2 on gel to do gel purification.
 +
<br>Only one of the tubes showed a band around the expected height.
 +
 +
<p>Made a double restriction digestion with fast restriction digest for Pdes and CheY with respectively EcoRI and BamHI; and BamHI and PstI.
 +
 +
 +
<h2>Chaline</h2>
 +
Made a ligation for the knockout system of Des, with Des UP, Tet and Des Down
 +
 +
<h2>Friso</h2>
 +
Made a restriction digestion for Spec purified.
 +
</div>
</div>

Revision as of 08:03, 29 July 2013

Mirjam

Run the samples of the colony PCR of BBa_K823823 combined with the LacI promoter made on 27-07 on gel (order 1-8).
There are no bands around 1500 bp.

Run the purified PCR product of spec on gel.
The purified spec shows a nice band.

Run the PCR products of silk 2 on gel to do gel purification.
Only one of the tubes showed a band around the expected height.

Made a double restriction digestion with fast restriction digest for Pdes and CheY with respectively EcoRI and BamHI; and BamHI and PstI.

Chaline

Made a ligation for the knockout system of Des, with Des UP, Tet and Des Down

Friso

Made a restriction digestion for Spec purified.