Team:Groningen/Labwork/2 August 2013

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<br>BBa_K823023 + lacI, BBa_E0840 and BBa_E0240 are digested with EcoRI and PstI.
<br>BBa_K823023 + lacI, BBa_E0840 and BBa_E0240 are digested with EcoRI and PstI.
The samples are purified using the PCR purification kit.
The samples are purified using the PCR purification kit.
 +
To the tube containing the purified vector 1&micro;l of SpeI and XbaI is added and the tube is incubated 1h at 37&deg;C.
BBa_K823023 + lacI is ligated to BBa_E0840 and BBa_E0240 (3:1 insert:vector ratio).
BBa_K823023 + lacI is ligated to BBa_E0840 and BBa_E0240 (3:1 insert:vector ratio).
The ligation products are transformed to E. Coli DH5α and the competent cells are plated on LB + Amp.  
The ligation products are transformed to E. Coli DH5α and the competent cells are plated on LB + Amp.  

Revision as of 10:24, 2 August 2013

Claudio

The plates prepared yesterday show colonies.

The strains inoculated by Inne grew. -80°C stock of AKP4 (Δdes::kn::cm) and OIB055 (ΔcheY::cm) was made and stored in the box labeled iGEM 2013.

BBa_K823023 + lacI, BBa_E0840 and BBa_E0240 are digested with EcoRI and PstI. The samples are purified using the PCR purification kit. To the tube containing the purified vector 1µl of SpeI and XbaI is added and the tube is incubated 1h at 37°C. BBa_K823023 + lacI is ligated to BBa_E0840 and BBa_E0240 (3:1 insert:vector ratio). The ligation products are transformed to E. Coli DH5α and the competent cells are plated on LB + Amp.