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Team:DTU-Denmark/Notebook/3 August 2013
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===Miniprep=== | ===Miniprep=== | ||
| - | Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using [[Team:DTU-Denmark/Methods/Plasmid_isolation|ethanol precipitation]]. | + | Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using [[Team:DTU-Denmark/Methods/Plasmid_isolation|ethanol precipitation]]. Actually should have used the Midiprep kit from QIAgen. |
| + | After ethanol precipitation continued with Midiprep kit step from step 9. | ||
==Results== | ==Results== | ||
Revision as of 14:23, 3 August 2013
3 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Gel electrophoresis
Run 1% agarose gel on overnight PCR products.
Miniprep
Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using ethanol precipitation. Actually should have used the Midiprep kit from QIAgen. After ethanol precipitation continued with Midiprep kit step from step 9.
Results
Gels
1% agarose gel
- 1kb ladder
- Nir1 high
- Nir1 high
- Nir1 low
- Nir1 low
- broad band ladder
- 43
- 43
- Nir2 GC
- Nir2 GC
- neg for Nir
- AMO
- AMO
- neg for AMO
- 1 kb ladder
Conclusion
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