Team:Paris Bettencourt/Notebook/Phage Sensor/Monday 15th July.html

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We made a 1:1000 dillution of KAN (stand.c) in an LB solid media.<br>
We made a 1:1000 dillution of KAN (stand.c) in an LB solid media.<br>
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Pooring the plates. Around 20 mL/plate<br>
Pooring the plates. Around 20 mL/plate<br>
1. KEIOΔPYR KANR Growth<br>
1. KEIOΔPYR KANR Growth<br>

Revision as of 15:25, 5 August 2013

Phage Sensor

Monday 15th July

Plating and control of antibiotic resistance.

We made a 1:1000 dillution of KAN (stand.c) in an LB solid media.

Pooring the plates. Around 20 mL/plate
1. KEIOΔPYR KANR Growth
2. ME1655 KANR No growth

Conclusion :

The KEIOΔPYRF growed as expected. Negative control did not growth.

Plates have KAN antibiotic
KEIO has KAN R.