"
Team:Paris Bettencourt/Notebook/Phage Sensor/Monday 29th July.html
From 2013.igem.org
(Difference between revisions)
Annelöchner (Talk | contribs) |
Annelöchner (Talk | contribs) |
||
| Line 16: | Line 16: | ||
pSSB2 (from plate ) - 5 ml culture<br> | pSSB2 (from plate ) - 5 ml culture<br> | ||
<br> | <br> | ||
| - | Plating of addgene bacteria containing plasmids:<br> | + | Plating of addgene bacteria containing the plasmids we want:<br> |
streak out bacteria from addgene to get single colonies:<br> | streak out bacteria from addgene to get single colonies:<br> | ||
pBAC-BA-lacZ<br> | pBAC-BA-lacZ<br> | ||
Revision as of 11:29, 6 August 2013
Phage Sensor
ASDFMonday 29th July
Liquid culture of:
pCOLA DUET (from glycerol stock of Trojan Horse Group) - 20 ml culture
pSSB1 (from plate) - 5ml culture
pSSB2 (from plate ) - 5 ml culture
Plating of addgene bacteria containing the plasmids we want:
streak out bacteria from addgene to get single colonies:
pBAC-BA-lacZ
pCRISPR
pCas9
pCRIPSR::rpsL
