Team:Groningen/Labwork/6 August 2013

From 2013.igem.org

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<br>The transformation of the positive candidate made last Saturday don't show any greenish colonies.
<br>The transformation of the positive candidate made last Saturday don't show any greenish colonies.
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<h2>Sebas</h2>
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Digestion check on BBa_K823832+lacI_hyperspank (our biobrick) with GFP (made by claudio):
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<br>image
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<br>1: undigested vector
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<br>2: Digestion with PstI*EcoRI expected size bands: ~900; ~7000
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<br>3: 1kb marker
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Revision as of 12:17, 6 August 2013

Mirjam

Inoculated ΔDes and ΔCheY in minimal medium.
Inoculated CFP in LB medium with cm.
Mini prep analysis of Pdes/CheY. Figured out why this is not working.
Made a restriction digestion of YFP/GFP with XbaI and PstI.
Made a restriction digestion of CheY with BamHI and SpeI.

Claudio

The plates screened last Saturday do not show any positive result. None of the colonies screened are greenish.

The transformation of the positive candidate made last Saturday don't show any greenish colonies.

Sebas

Digestion check on BBa_K823832+lacI_hyperspank (our biobrick) with GFP (made by claudio):
image
1: undigested vector
2: Digestion with PstI*EcoRI expected size bands: ~900; ~7000
3: 1kb marker