Team:DTU-Denmark/Notebook/6 August 2013

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(Results)
(Procedure)
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==Procedure==
==Procedure==
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===PCR===
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lots of PCR done
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===gel purification===
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of the Nir1 fragment extraced from P. aeruginosa
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===plasmid miniprep===
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HAO in pZA21 from USER cloning was purified for restriction analysis
==Results==
==Results==

Revision as of 15:01, 6 August 2013

6 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Gosia, Natalia, Henrike

Procedure


PCR

lots of PCR done

gel purification

of the Nir1 fragment extraced from P. aeruginosa

plasmid miniprep

HAO in pZA21 from USER cloning was purified for restriction analysis

Results


1% agarose gel

  • 1 kb ladder
  • Nir1 col. T
  • Nir1 col. T
  • Nir2 col. T
  • Nir2 col. T
  • Nir1 frag. T
  • Nir1 frag. T
  • Nir1 col. X7
  • Nir1 col. X7
  • neg
  • Hi
  • Hi
  • DMSO
  • DMSO
  • Nir1
  • Nir1
  • Nir2
  • Nir2
  • 1 kb ladder

2013-08-06 nir.jpg

1% agarose gel

  • 1 kb ladder
  • NirW
  • NirW
  • araBAD biobrick K808000
  • araBAD biobrick K808000
  • SPL in pZA21 containing RFP
  • SPL in pZA21 containing RFP
  • reference promoter in pZA21 containing RFP
  • reference promoter in pZA21 containing RFP
  • neg
  • neg
  • 1 kb ladder

(pic small gel)

Conclusion


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