Team:Bielefeld-Germany/Labjournal/Molecular

From 2013.igem.org

(Difference between revisions)
Line 20: Line 20:
       <col width="50">
       <col width="50">
       <col width="50">
       <col width="50">
-
<tr><td>Reagenz</td> <td>1x</td> <td>8</td> </tr>
+
<tr><th>Reagenz</th> <th>1x</th> <th>8</th> </tr>
<td>Buffer 5x</td> <td>5,0</td> <td>44</td>
<td>Buffer 5x</td> <td>5,0</td> <td>44</td>
-
MgSO4 0
+
<td>MgSO4</td> <td>0</td>      <td>0</td>
-
dNTPs 2,5 22
+
<td>dNTPs</td>  <td>2,5</td> <td>22</td>
-
Primer1 1,0 8,8
+
<td>Primer1</td> <td>1,0</td> <td>8,8</td>
-
Primer2 1,0 8,8
+
<td>Primer2</td> <td>1,0</td> <td>8,8</td>
-
Template 1,5 13,2
+
<td>Template</td> <td>1,5</td> <td>13,2</td>
-
DMSO 2,0 17,6
+
<td>DMSO</td>         <td>2,0</td>    <td>17,6</td>
-
Polymerase 0,5 4,4
+
<td>Polymerase</td> <td>0,5</td> <td>4,4</td>
-
H2O 11,5 101,2
+
<td>H2O</td>    <td>11,5</td> <td>101,2</td>
</table>
</table>
</html>
</html>

Revision as of 22:43, 6 August 2013




PCR Protocols and Programs

Phusion 25 µl

Reagenz 1x 8
Buffer 5x 5,0 44 MgSO4 0 0 dNTPs 2,5 22 Primer1 1,0 8,8 Primer2 1,0 8,8 Template 1,5 13,2 DMSO 2,0 17,6 Polymerase 0,5 4,4 H2O 11,5 101,2