"

From 2013.igem.org

Revision as of 22:52, 6 August 2013 (view source) Sgrenz (Talk | contribs) ← Older edit		Revision as of 22:54, 6 August 2013 (view source) Sgrenz (Talk | contribs) Newer edit →
Line 40:		Line 40:
	<col width="50">		<col width="50">
	<tr><th colspan="5">Program Phusion</th></tr>		<tr><th colspan="5">Program Phusion</th></tr>
-	<tr><td>98°C</td><td>98°C</td><td>70°C</td><td>72°C</td><td>72°C</td></tr>	+	<tr><td>98°C</td><td>98°C</td><td>x°C</td><td>72°C</td><td>72°C</td></tr>
-	<tr><td>2min</td> <td>30s</td><td>30s</td> <td>45s</td><td>6min</td></tr>	+	<tr><td>2min</td> <td>30s</td><td>30s</td> <td>y</td><td>6min</td></tr>
-		+	<tr>x: according to Primer Tm    y: 15s/kb</tr>
	</table>		</table>
	</html>		</html>

---

Revision as of 22:54, 6 August 2013

PCR Protocols and Programs

Phusion 25 µl

Reagenz	1x	8x
Buffer 5x	5,0	44
MgSO4	0	0
dNTPs	2,5	22
Primer1	1,0	8,8
Primer2	1,0	8,8
Template	1,5	13,2
DMSO	2,0	17,6
Polymerase	0,5	4,4
H2O	11,5	101,2

x: according to Primer Tm y: 15s/kb

Program Phusion
98°C	98°C	x°C	72°C	72°C
2min	30s	30s	y	6min