Team:Bielefeld-Germany/Labjournal/Molecular

From 2013.igem.org

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<tr><th colspan="5">Program Phusion</th></tr>
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<tr><th colspan="5">Program Taq</th></tr>
<tr><td>98 °C</td><td>98 °C</td><td>x °C</td><td>72 °C</td><td>72 °C</td></tr>
<tr><td>98 °C</td><td>98 °C</td><td>x °C</td><td>72 °C</td><td>72 °C</td></tr>
<tr><td>2 min</td> <td>30 s</td><td>30 s</td> <td>y</td><td>6 min</td></tr>
<tr><td>2 min</td> <td>30 s</td><td>30 s</td> <td>y</td><td>6 min</td></tr>

Revision as of 23:00, 6 August 2013




PCR Protocols and Programs

Phusion 25 µl

Reagenz 1x 8x
Buffer 5x 5,0 44
MgSO4 0 0
dNTPs 2,5 22
Primer1 1,0 8,8
Primer2 1,0 8,8
Template 1,5 13,2
DMSO 2,0 17,6
Polymerase0,5 4,4
H2O 11,5 101,2
Program Phusion
98 °C98 °Cx °C72 °C72 °C
2 min 30 s30 s y6 min
x: according to Primer Tm
y: 15s/kb
















Taq 25 µl

Reagenz 1x 8x
Buffer 5x 5,0 44
MgSO4 0 0
dNTPs 2,5 22
Primer1 1,0 8,8
Primer2 1,0 8,8
Template 1,5 13,2
DMSO 2,0 17,6
Polymerase0,5 4,4
H2O 11,5 101,2
Program Taq
98 °C98 °Cx °C72 °C72 °C
2 min 30 s30 s y6 min
x: according to Primer Tm
y: 15s/kb

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