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Revision as of 12:52, 7 August 2013 (view source) ChristopherW (Talk | contribs) (Created page with "===Tag and binding sites=== *Arg5-tag: RRRRR *''Strep''-tag I: AWRHPQFGG *''Strep''-tag II: WSHPQFEK *Flag-tag: DYKDDDDK *3xFlag-tag: DYKDHDGDYKDHDIDYKDDDDK *HA-tag: YPYDVPDYA ...") ← Older edit		Revision as of 12:52, 7 August 2013 (view source) ChristopherW (Talk | contribs) (→Cleavage sites) Newer edit →
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	===Cleavage sites===		===Cleavage sites===
-	Enterokinase cleavage site: DDDDK	+	*Enterokinase cleavage site: DDDDK
-	Factor Xa cleavage site: ID/EGR	+	*Factor Xa cleavage site: ID/EGR
-	Thrombin cleavage site: LVPRGS	+	*Thrombin cleavage site: LVPRGS
-	PreScission cleavage site: LEVLFQGP	+	*PreScission cleavage site: LEVLFQGP
-	TEV cleavage site: ENLYFQG	+	*TEV cleavage site: ENLYFQG
-	TVMV cleavage site: ETVRFQGS	+	*TVMV cleavage site: ETVRFQGS

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Revision as of 12:52, 7 August 2013

Tag and binding sites

• Arg5-tag: RRRRR
• Strep-tag I: AWRHPQFGG
• Strep-tag II: WSHPQFEK
• Flag-tag: DYKDDDDK
• 3xFlag-tag: DYKDHDGDYKDHDIDYKDDDDK
• HA-tag: YPYDVPDYA
• His5-tag: HHHHH
• His6-tag: HHHHHH
• c-Myc-tag: EQKLISEEDL
• S-tag: KETAAAKFERQHMDS
• HAT-tag: KDHLIHNVHKEFHAHAHNK
• Calmodulin-binding-peptide: KRRWKKNFIAVSAANRFKKISSSGAL
• Isopep-tag: TDKDMTITFTNKKDAE
• Spy-tag: AHIVMVDAYKPTK
• Chitin-binding domain: TNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNVPALWQLQ
• SBP-tag: MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP
• Avitag: GLNDIFEAQKIEWHE

Cleavage sites

• Enterokinase cleavage site: DDDDK
• Factor Xa cleavage site: ID/EGR
• Thrombin cleavage site: LVPRGS
• PreScission cleavage site: LEVLFQGP
• TEV cleavage site: ENLYFQG
• TVMV cleavage site: ETVRFQGS