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Exeter/7 July 2013
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==Making standard liquid cultures of our transformed cells== | ==Making standard liquid cultures of our transformed cells== | ||
| - | In each 10 ml Falcon, we need | + | In each 10 ml Falcon, we need: |
- 5 ml LB broth | - 5 ml LB broth | ||
| Line 7: | Line 7: | ||
- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone) | - 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone) | ||
| - | We used a 50 ml conical flask of LB broth which had been autoclaved and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the | + | We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes. |
| - | + | We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube. | |
| - | The tubes | + | The tubes were incubated overnight in a spinning 37oC incubator. |
Revision as of 15:04, 7 August 2013
Making standard liquid cultures of our transformed cells
In each 10 ml Falcon, we need:
- 5 ml LB broth
- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes.
We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube.
The tubes were incubated overnight in a spinning 37oC incubator.
