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Exeter/7 July 2013
From 2013.igem.org
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We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube. | We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube. | ||
| - | The tubes were incubated overnight in a spinning | + | The tubes were incubated overnight in a spinning 37°C incubator. |
Revision as of 15:05, 7 August 2013
Making standard liquid cultures of our transformed cells
In each 10 ml Falcon, we need:
- 5 ml LB broth
- 5 ul antibiotic (in this case, all of our transformed cells are on the pSB1C3 backbone)
We used a 50 ml conical flask of LB broth which had been autoclaved, and added 50 ul of chloramphenicol to make a "stock" to be pipetted into the Falcon tubes.
We then stabbed the chosen colony with a pipette tip and ejected it into the Falcon tube.
The tubes were incubated overnight in a spinning 37°C incubator.
