Team:ITB Indonesia

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<title>Ehime-Japan iGEM Team: Welcome</title>
 
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<p  style="text-align:center;"><a href="https://2012.igem.org/Team:Ehime-Japan"><img src="https://static.igem.org/mediawiki/2012/b/b4/Ecolingual%E3%82%A6%E3%82%A3%E3%82%AD%E7%94%A8%EF%BC%92.png" width="800px"></a></p>
 
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan">Home</a></li> 
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Project">Project</a>
 
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#one">E.co-mail</a></li>
 
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#two">E.co-Domino</a></li>
 
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                        <li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#three">E.cold-heat sensing system</a></li>
 
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<li><A HREF="https://2012.igem.org/Team:Ehime-Japan/Project#four">E.colingual</a></li>
 
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        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Modeling">Modeling</a></li>
 
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<li><a href="#">Data</a>
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Parts">BioBrick parts</a></li>
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PlasmidsAndStrain">Plasmids And Strain</a></li>
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/PrimerList">Primer List</a></li>
 
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<li><a href="#">Human Practice</a>
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create">E.create</a></li>
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire">Questionnaire</a></li>
 
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<li><a href="#">Other</a>
 
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<li><a href="https://2012.igem.org/Team:Ehime-Japan/Team">team</a></li>
 
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<li><a href="https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan">team profile</a></li>
 
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Notebook">note book</a></li>
 
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Safety">safety</a></li>
 
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                        <li><a href="https://2012.igem.org/Team:Ehime-Japan/Attributions">attribution</a></li> 
 
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Ehime-Japan team challenged to three projects, which would finally lead us to create “E.colingual”, a tool through which we can know <i>E. coli</i> feelings. It consists of three parts: Main, Connection, and Screen.<br>
 
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To realize our project, we used the light sensor genes from <i>Synechocystis</i>, the Lon protease and the <i>ssrA</i> tag from <i>Mesoplasma florum</i>. Also, a cell-cell signaling system and the heat shock and cold shock promoters were used so as to construct a foundational system of E.colingual.
 
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“E.co-mail” is a communication tool with <i>E. coli</i> and an optical fiber. We would be able to transfer digital information depicted in <i>E.coli</i> through a distance, and probably could send e-mails with this tool. 
 
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a>
 
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<img src="https://static.igem.org/mediawiki/2012/9/9d/Eco-Domino.png" width=700px><br><br>
 
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    We tried to reconstruct toppling dominoes with <i>E. coli</i>,
 
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    and we would be able to draw a movie of firework on LB plate!
 
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    Main is comprised of two different kinds of <i>E. coli</i> transformants.
 
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    One is that feels heat and cold and respond differently, producing different
 
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    signaling molecules. The other catches the signaling molecules and convert
 
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    the signal into the form of light. As a result, we can see a temperature change
 
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    around the <i>E. coli</i>. The light signals could be transferred through a distance
 
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    and be displayed if the Connection and Screen systems work.
 
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#three"><p><font color=blue>See more…</font color><p></a>
 
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https://static.igem.org/mediawiki/2012/9/93/E.colingual.png" width=800px><br><br>
 
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    It consists of three parts:Main, Connection, and Screen. E.colingual is a tool through which
 
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    we can know <i>E. coli</i> feelings:“hot”,“cold”, “happy” ,or “dying” etc…
 
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    Also, by an optical fiber, the information of those feelings is transmitted
 
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    to someone in a distant place from a lab. Actually, we focused on <i>E. coli</i>
 
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    feelings, “hot” and “cold”.
 
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→  New degradation system
 
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    We used two different <i>ssrA</i> tags to make a circulatable communication system. One is from
 
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    <i>M. florum</i> recognized by the Lon protease from <i>M. florum</i>. The other is <i>E. coli ssrA</i> tag (LVA).
 
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    Because the sequences of those tags are not similar, the degradation systems should function
 
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    independently.     
 
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<a href="https://2012.igem.org/Team:Ehime-Japan/Project#one"><p><font color=blue>See more…</font color><p></a>
 
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→ New parts
 
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    We made new BioBrick parts!
 
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  ・BBa_K794000 : This part is cold shock promoter + <i>lasI</i>.
 
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<a href="https://2012.igem.org/Team:Ehime-Japan/Parts"><p><font color=blue>See more…</font color><p></a>
 
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→ Human Practice
 
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Ehime-Japan did a lot of projects as Human Practice!
 
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・Ecreate !
 
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    We are enjoying gene recombination, and to tell interests of synthetic biology for
 
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    high school students, we made “E.create”!! It is a card game that people can enjoy
 
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    building genetic circuits. Also, we did educational activities for high school students
 
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    and played them with E.create! 
 
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<a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-E.create"><p><font color=blue>See more…</font color><p></a>
 
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・Questionnaire
 
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    Our survey was carried out by Ehime-Japan, Osaka and KIT-Kyoto iGEM 2012 teams together!
 
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    Thus, we were able to obtain a lot of replies, and found more general opinions
 
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    about synthetic biology in Japan.
 
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<a href="https://2012.igem.org/Team:Ehime-Japan/HumanPractice-Questionnaire"><p><font color=blue>See more…</font color><p></a>
 
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<!--- The Mission, Experiments --->
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:Ehime-Japan|Home]]
 
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!align="center"|[[Team:Ehime-Japan/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Ehime-Japan Official Team Profile]
 
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!align="center"|[[Team:Ehime-Japan/Project|Project]]
 
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!align="center"|[[Team:Ehime-Japan/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:Ehime-Japan/Modeling|Modeling]]
 
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!align="center"|[[Team:Ehime-Japan/Notebook|Notebook]]
 
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!align="center"|[[Team:Ehime-Japan/Safety|Safety]]
 
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!align="center"|[[Team:Ehime-Japan/Attributions|Attributions]]
 
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|}
 

Revision as of 22:43, 20 May 2013

Retrieved from "http://2013.igem.org/Team:ITB_Indonesia"