Team:Glendale CC AZ/Protocols/SurivalGrowth

From 2013.igem.org

(Difference between revisions)
Line 19: Line 19:
1. Label all plates (Keep upside down to avoid condensation on agar)
1. Label all plates (Keep upside down to avoid condensation on agar)
 +
2. Pipette LB media into tube containing bacteria; aspirate.
2. Pipette LB media into tube containing bacteria; aspirate.
-
3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate.  
+
 
-
4. Briefly flame the spreader and let cool momentarily.  
+
3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate.
-
5. Spread bacteria-LB solution all over plate.  
+
 
 +
4. Briefly flame the spreader and let cool momentarily.
 +
 
 +
5. Spread bacteria-LB solution all over plate.
 +
 
6. Incubate at 37 degrees celcius for up to 24 hours.
6. Incubate at 37 degrees celcius for up to 24 hours.

Revision as of 19:25, 11 August 2013


Survival Growth Assay

Materials 

   -P200 micropipette
   -10 microliters bacteria
   -90 mictroliters LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37 degrees celcius.


Procedure

1. Label all plates (Keep upside down to avoid condensation on agar)

2. Pipette LB media into tube containing bacteria; aspirate.

3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate.

4. Briefly flame the spreader and let cool momentarily.

5. Spread bacteria-LB solution all over plate.

6. Incubate at 37 degrees celcius for up to 24 hours.

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