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Team:Glendale CC AZ/Protocols/SurivalGrowth
From 2013.igem.org
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-P200 micropipette | -P200 micropipette | ||
| - | - | + | -10µl bacteria |
| - | - | + | -90µl LB media |
-Agar plates with appropriate antibiotic | -Agar plates with appropriate antibiotic | ||
-Spreader | -Spreader | ||
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2. Pipette LB media into tube containing bacteria; aspirate. | 2. Pipette LB media into tube containing bacteria; aspirate. | ||
| - | 3. Using P200 micropipette, extract all | + | 3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate. |
4. Briefly flame the spreader and let cool momentarily. | 4. Briefly flame the spreader and let cool momentarily. | ||
Revision as of 22:48, 11 August 2013
Survival Growth Assay
Materials
-P200 micropipette -10µl bacteria -90µl LB media -Agar plates with appropriate antibiotic -Spreader -20mL ethanol (to sterilize spreader) -Incubator set to 37 degrees celcius.
Procedure
1. Label all plates (Keep upside down to avoid condensation on agar)
2. Pipette LB media into tube containing bacteria; aspirate.
3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.
4. Briefly flame the spreader and let cool momentarily.
5. Spread bacteria-LB solution all over plate.
6. Incubate at 37 degrees celcius for up to 24 hours.
