Team:BostonU/Protocols

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<p  style="text-align:center;"><a href="https://2013.igem.org/Team:BostonU"><img src="https://static.igem.org/mediawiki/2013/2/2c/Marsh.png" width="800px" length="200px"></a></p>
 
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<li><a href="#">Our Team</a>
 
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<ul>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Team">Team</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Social">Summer Fun</a></li>
 
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<li><a href="#">Project</a>
 
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<ul>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/MoCloChara">Introduction to MoClo and Characterization</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/QS">Quorum Sensing</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/HK">Histidine Kinase</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/ML">MoClo Library</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Results ">Results Summary</a></li>
 
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<li><a href="#">Achievements</a>
 
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<ul>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Data">Data Collected</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>
 
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</ul>
 
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</li>
 
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<li><a href="#">Notebook</a>
 
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<ul>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Protocols">Protocols</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Clotho">Clotho and Eugene</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/PR">Pigeon and Raven</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/NotebookQS">Quorum Sensing Notebook</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookHK">Histidine Kinase Notebook</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookML">MoClo Library Notebook</a></li>
 
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                        <li><a href="https://2013.igem.org/Team:BostonU/TroubleShooting">Trouble Shooting</a></li>
 
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</li>
 
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<li><a href="#">Considerations</a>
 
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<ul>
 
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<li><a href="https://2013.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
 
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<li><a href="https://2013.igem.org/Team:BostonU/Safety">Safety</a></li>
 
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</ul>
 
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<li><a href="#">Acknowledgements</a>
 
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<ul>
 
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                    <li><a href="https://2013.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
 
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                    <li><a href="https://2013.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
 
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                </ul>
 
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Revision as of 17:34, 23 May 2013




Protocols

<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook.</p> We hope they help anyone interested in our experiments to successfully repeat them.


  • <a href="https://static.igem.org/mediawiki/2012/0/0b/Phusion_PCRblank.xls">Phusion PCR </a></p>
  • <a href="https://static.igem.org/mediawiki/2012/c/cb/Transformation_Gold_Bioline_cells.xls">Transformation with Gold Bioline Cells</a></p>
  • <a href="https://static.igem.org/mediawiki/2012/0/05/Restriction_Digest.xls">Restriction Digest </a></p>
  • <a href="https://static.igem.org/mediawiki/2012/8/8e/Qiagen_QIAquick_Gel_extraction.xls">Gel Extraction </a></p>
  • <a href="https://static.igem.org/mediawiki/2012/5/58/Qiagen_QIAprep_Miniprep.xls">Miniprep </a></p>
  • <a href="https://static.igem.org/mediawiki/2012/7/7e/NanoDrop_DNA_Quantification.xls">DNA Quantification </a></p>
  • <a href="https://static.igem.org/mediawiki/2012/b/bd/Media_Preparation_LB_broth_agar_Gels.xls">Media Prep </a></p>
  • <a href="https://static.igem.org/mediawiki/2012/1/1f/Ligation_Regular_and_MoClo.xls">Ligation </a></p> </html>