Revision as of 21:21, 12 August 2013

Notebook : August 12

Obtaining Pfnr_RBS-LacZ-Term in PSB1C3

Anaïs, Nadia, XiaoJing

Protocol : digestion

Used quantities :

We gonna check if the digestion was good by control the size of digestion pieces.

Expected sizes :

We can't see any band for Bba_K1155000 digestion. We just do it again and let it in the fridge over night.

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@@ Line 58: / Line 58: @@
 ...
-===='''1 - Digestion of Bba_K1155000, Bba_K11'''====
-Abdou
-Clone 10,14 and 15 plamid extraction using nucleospin kit.
-Protocol : [[Team:Paris_Saclay/Protocols/hight copy plamid extraction|hight copy plamid extraction]]
-Results: concentration measured by nanodrop
-*clone 10: C=38ng/µl   260/280= 1.78
-*clone 14: C=48.5ng/µl   260/280=1.90
-*clone 15: C=52 ng/µl     260/280=1.78
-We still have to sequence plasmids in order to verify our results.
 {{Team:Paris_Saclay/incl_fin}}