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Team:Paris Saclay/Notebook/August/12
From 2013.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
Used quantities : | Used quantities : | ||
* Pfnr in PSB1C3 (Bba_K1155000) : | * Pfnr in PSB1C3 (Bba_K1155000) : | ||
| - | ** Buffer : 5µL | + | ** Buffer FD : 5µL |
** H2O : 38µL | ** H2O : 38µL | ||
** Pfnr : 5µL | ** Pfnr : 5µL | ||
| Line 24: | Line 24: | ||
* RBS-LacZ-Term in PSB1C3 (Bba_K1155007) : | * RBS-LacZ-Term in PSB1C3 (Bba_K1155007) : | ||
| - | ** Buffer : 5µL | + | ** Buffer FD : 5µL |
** H2O : 23µL | ** H2O : 23µL | ||
** RBS-LacZ-Term : 20µL | ** RBS-LacZ-Term : 20µL | ||
| Line 31: | Line 31: | ||
* RBS-AmilCP-Term in PSB1C3 (Bba_K1155003) : | * RBS-AmilCP-Term in PSB1C3 (Bba_K1155003) : | ||
| - | ** Buffer : 5µL | + | ** Buffer FD : 5µL |
** H2O : 33µL | ** H2O : 33µL | ||
** RBS-AmilCP-Term : 10µL | ** RBS-AmilCP-Term : 10µL | ||
Revision as of 21:49, 12 August 2013
Contents |
Notebook : August 12
Lab work
A - Aerobic/Anaerobic regulation system
Obtaining Pfnr_RBS-LacZ-Term in PSB1C3
1 - Digestion of Bba_K1155000, Bba_K1155007 and Bba_K1155003
Anaïs, Nadia, XiaoJing
Protocol : digestion
Used quantities :
- Pfnr in PSB1C3 (Bba_K1155000) :
- Buffer FD : 5µL
- H2O : 38µL
- Pfnr : 5µL
- SpeI : 1µL
- PstI : 1µL
- RBS-LacZ-Term in PSB1C3 (Bba_K1155007) :
- Buffer FD : 5µL
- H2O : 23µL
- RBS-LacZ-Term : 20µL
- XBal : 1µL
- PstI : 1µL
- RBS-AmilCP-Term in PSB1C3 (Bba_K1155003) :
- Buffer FD : 5µL
- H2O : 33µL
- RBS-AmilCP-Term : 10µL
- XBal : 1µL
- PstI : 1µL
2 - Electrophoresis of digestion pieces
We gonna check if the digestion was good by control the size of digestion pieces.
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Expected sizes :
- RBS_LacZ_Term : 3500 kb
- RBS_AmilCP_Term :
- PSB1C3 :
We can't see any band for Bba_K1155000 digestion. We just do it again and let it in the fridge over night.
...
