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Team:DTU-Denmark/Methods/Making anoxic conditions
From 2013.igem.org
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# Open the pressure valve of the Nitrogen gas container | # Open the pressure valve of the Nitrogen gas container | ||
# Turn round (anticlockwise) the nitrogen valve to open it until there is a flow of gas. | # Turn round (anticlockwise) the nitrogen valve to open it until there is a flow of gas. | ||
| - | # Sparge with | + | # Sparge with N<sub>2</sub> by connecting the N<sub>2</sub> gas tube with the long needle. |
# After 3/ 5 min remove first the small needle and after 2 seconds the long needle. | # After 3/ 5 min remove first the small needle and after 2 seconds the long needle. | ||
# Turn off the nitrogen valve. | # Turn off the nitrogen valve. | ||
Revision as of 08:59, 13 August 2013
Sparging with N2
Sparging with N2
Method: Injection of N2 to achieve anaerobic environment
- Procedure
- Put carefully one long needle in the vessel for injecting N2
- Put one short needle in the vessel for removing the containing oxygen
- Open the pressure valve of the Nitrogen gas container
- Turn round (anticlockwise) the nitrogen valve to open it until there is a flow of gas.
- Sparge with N2 by connecting the N2 gas tube with the long needle.
- After 3/ 5 min remove first the small needle and after 2 seconds the long needle.
- Turn off the nitrogen valve.
- Turn off the pressure valve.
