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UIUC Illinois/Safety/Database
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==Safety Database== | ==Safety Database== | ||
| - | + | ==EtBr== | |
| - | + | [www.purdue.edu/rem/hmm/ethidbr.htm] | |
| - | + | *Ethidium bromide(EtBR) is a known toxin and mutagen. Its use should be limited and it should be handled with EXTREME CARE. | |
| - | + | *Only use EtBr in designated areas clearly marked “EtBr contamination” to avoid contaminating other lab areas and equipment. | |
| - | + | *Any PPE that has been contaminated with EtBr must be disposed of in an EtBr container | |
| - | + | **Latex gloves should not be worn when working with EtBr. Nitrile gloves are sufficient when minimal exposure time is expected. For longer periods of time, use multiple layers or chemically-resistant gloves. | |
| - | + | **Wash hands thoroughly and immediately after disposing of gloves | |
| + | *If accidental exposure occurs: | ||
| + | **Eyes- rinse with water for 15 minutes | ||
| + | **Skin- wash the contaminated area with soap and water | ||
| + | **Clothing- remove and place in sealed bag | ||
| + | **ALWAYS seek medical attention immediately after an accident has occurred | ||
| + | *Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr | ||
| + | ==Solid Disposal== | ||
| + | ==Liquid Disposal== | ||
| + | ==General decontamination== | ||
| + | ==Electrophoresis machine== | ||
| + | ==Proper Bunsen burner technique== | ||
| + | ==Appropriate PPE== | ||
| + | |||
| + | |||
| + | |||
| + | <!-- Resource: http://www.mediawiki.org/wiki/Help:Contents Honestly I just follow this to a T and it is quite easy to create a wikipedia page so go ahead and edit as you like :) --> | ||
Revision as of 13:28, 13 August 2013
Contents |
Safety Database
EtBr
[www.purdue.edu/rem/hmm/ethidbr.htm]
- Ethidium bromide(EtBR) is a known toxin and mutagen. Its use should be limited and it should be handled with EXTREME CARE.
- Only use EtBr in designated areas clearly marked “EtBr contamination” to avoid contaminating other lab areas and equipment.
- Any PPE that has been contaminated with EtBr must be disposed of in an EtBr container
- Latex gloves should not be worn when working with EtBr. Nitrile gloves are sufficient when minimal exposure time is expected. For longer periods of time, use multiple layers or chemically-resistant gloves.
- Wash hands thoroughly and immediately after disposing of gloves
- If accidental exposure occurs:
- Eyes- rinse with water for 15 minutes
- Skin- wash the contaminated area with soap and water
- Clothing- remove and place in sealed bag
- ALWAYS seek medical attention immediately after an accident has occurred
- Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr
