Team:NTNU-Trondheim/Safety

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Revision as of 09:11, 14 August 2013

Safety

The university has a health, safety and environment (HSE) group with set guidelines and rules to regulate the work environment and safety. General information about this can be found at http://www.ntnu.edu/hse. Details on the HSE regulations for lab work can be found at http://www.ntnu.edu/hse/labhandbook. These are all based on the national biosafety regulations and guidelines set by the Norwegian Biotechnology Advisory Board (http://www.bion.no/english/norwegian-regulation) and the acts set by the government.

Lab work

The institute for Biotechnology has set some local guidelines and rules that apply for the specific labs that we use in our lab work for this project. The labs used are approved for work with gene-modified organisms (GMOs). Prior to the commencement of work in any of these labs, every person is given the essential training. The training covers the statutory regulations and safety provisions, the use of equipment and protective equipment, fire extinguishing, first aid, use of the chemical substances index and safety data sheets, proper waste disposal and reporting. The wetlab part of the NTNU iGEM team has all attended this training and is following the set lab guidelines and rules in their work.

We of course follow general lab safety and wear lab coats, disposable gloves and safety glasses when required by the safety guidelines and rules. The materials used in this project are mostly standard kits, restriction enzymes and buffers that are bought and come pre-made from biotechnology companies, and does not require any other safety precautions than the general ones already described above. This also goes for the preparation of solutions like growth medium and gels for gel electrophoresis. When doing gel electrophoresis we use GelRed instead of ethidium bromide, as it is said not to penetrate cell membranes and thus should not be able to work as a mutagen. However, to be on the safe side, we always wear nitrile gloves when working with GelRed.

Use this page to answer the questions on the safety page.

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 <p> The institute for Biotechnology has set some local guidelines and rules that apply for the specific labs that we use in our lab work for this project. The labs used are approved for work with gene-modified organisms (GMOs). Prior to the commencement of work in any of these labs, every person is given the essential training. The training covers the statutory regulations and safety provisions, the use of equipment and protective equipment, fire extinguishing, first aid, use of the chemical substances index and safety data sheets, proper waste disposal and reporting. The wetlab part of the NTNU iGEM team has all attended this training and is following the set lab guidelines and rules in their work.</p>
+<p> We of course follow general lab safety and wear lab coats, disposable gloves and safety glasses when required by the safety guidelines and rules. The materials used in this project are mostly standard kits, restriction enzymes and buffers that are bought and come pre-made from biotechnology companies, and does not require any other safety precautions than the general ones already described above. This also goes for the preparation of solutions like growth medium and gels for gel electrophoresis. When doing gel electrophoresis we use GelRed instead of ethidium bromide, as it is said not to penetrate cell membranes and thus should not be able to work as a mutagen. However, to be on the safe side, we always wear nitrile gloves when working with GelRed.
+</p>