15/08/13

(Difference between revisions)

Latest revision as of 13:29, 15 August 2013

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100ul DNA and 900ul TE to make master mix
5 independent samples 10ul each were taken from master mix
- Each independent sample was diluted twice in 90ul of TE
20 dilution samples were then nanodropped to check dilution accuracy

Dilutions show accuracy
Master dilution is sonicated and every 30mins a 30ul sample is taken to dilute twice (10ul of DNA in 90ul of TE buffer)
6 30ul samples are taken
Samples are then run on a gel

@@ Line 17: / Line 17: @@
 *100ul DNA and 900ul TE to make master mix
-*5 independent samples
+*5 independent samples 10ul each were taken from master mix
+**Each independent sample was diluted twice in 90ul of TE
+*20 dilution samples were then nanodropped to check dilution accuracy
+{|border=1
+|Sample nr||Concentration (ng/ul)||260/280||260/230
+|-
+|nr. 1/1||529.9||1.81||2.26
+|-
+|nr. 1/2||52.1||1.86||2.32
+|-
+|nr. 2/1||454.3||1.81||2.28
+|-
+|nr. 2/2||46||1.82||2.23
+|-
+|nr. 3/1||457.3||1.83||2.28
+|-
+|nr. 3/2||45.1||1.85||2.38
+|-
+|nr. 4/1||522.8||1.82||2.27
+|-
+|nr. 4/2||47.3||1.87||2.26
+|-
+|nr. 5/1||481.4||1.82||2.28
+|-
+|nr. 5/2||40.8||1.84||2.35
+|}
+*Dilutions show accuracy
+*Master dilution is sonicated and every 30mins a 30ul sample is taken to dilute twice (10ul of DNA in 90ul of TE buffer)
+*6 30ul samples are taken
+*Samples are then run on a gel