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Team:DTU-Denmark/Notebook/15 August 2013
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==Results== | ==Results== | ||
<hr/> | <hr/> | ||
| + | |||
| + | * 1 kb ladder | ||
| + | * purification of the extraction fragment of Nir2 | ||
| + | * Nir2 USER touchdown, sample 1 | ||
| + | * Nir2 USER touchdown, sample 2 | ||
| + | * Nir2 USER touchdown, sample 3 | ||
| + | * Nir2 USER touchdown, sample 4 | ||
| + | * Nir2 USER touchdown, sample 5 | ||
| + | * Nir2 USER touchdown negative | ||
| + | * 1 kb ladder | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 14:12, 15 August 2013
15 August 2013
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Contents |
lab 208
Main purpose
- Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBAD::RFP.
Who was in the lab
Ariadni, Helen, Kristian, Julia
Procedure
Plasmid isolation
Plasmid have been isolated following the protocol provided by ORIGENE PowerPrep HP Plasmid Miniprep Kit.
Glycerol stock cultures
-80C stock solution of Sec2, TAT3-1a, TAT3-2
Results
- 1 kb ladder
- purification of the extraction fragment of Nir2
- Nir2 USER touchdown, sample 1
- Nir2 USER touchdown, sample 2
- Nir2 USER touchdown, sample 3
- Nir2 USER touchdown, sample 4
- Nir2 USER touchdown, sample 5
- Nir2 USER touchdown negative
- 1 kb ladder
Conclusion
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